Induction of endothelin-1 gene by angiotensin and vasopressin in endothelial cells

Taihei Imai, Yukio Hirata, Toshiaki Emori, Masashi Yanagisawa, Tomoh Masaki, Fumiaki Marumo

Research output: Contribution to journalArticle

325 Citations (Scopus)

Abstract

To elucidate the cellular mechanism of endothelin-1 biosynthesis induced by angiotensin and vasopressin, we first cloned and sequenced full-length bovine preproendothelin-1 complementary DNA (cDNA) from a cultured bovine carotid artery endothelial cell cDNA library. The predicted bovine preproendothelin-1 consists of 202 amino acid residues and has a high percentage of homology to human, porcine, and rat preproendothelin-1 (70%, 81%, and 77%, respectively). Big endothelin-1, an intermediate form, consists of 39 residues differing only at position Val28 from porcine (Ile28) and His27 from rat (Arg27). The predicted 21-residue mature endothelin-1 is identical to human, porcine, rat, canine, and mouse endothelin-1. Northern blot analysis with the cloned cDNA as a probe demonstrated that a single 2.3-kb preproendothelin-1 messenger RNA (mRNA) is expressed not only in endothelial cells, but also in various bovine tissues, including lung, brain, heart, intestine, kidney, ovary, and urinary bladder. Angiotensin II and arginine vasopressin immediately and dose-dependently induced expression of preproendothelin-1 mRNA, whose effects were abolished by specific receptor antagonists. These findings suggest that stimulation of endothelin-1 secretion from endothelial cells by both agonists may be principally due to induction of preproendothelin-1 mRNA.

Original languageEnglish (US)
Pages (from-to)753-757
Number of pages5
JournalHypertension
Volume19
Issue number6 PART 2
StatePublished - Jun 1992

Fingerprint

Angiotensins
Endothelin-1
Vasopressins
Endothelial Cells
Genes
Swine
Complementary DNA
Messenger RNA
Arginine Vasopressin
Gene Library
Carotid Arteries
Angiotensin II
Northern Blotting
Intestines
Canidae
Ovary
Urinary Bladder
Kidney
Amino Acids
Lung

Keywords

  • Angiotensins
  • Cloning, molecular
  • DNA
  • Endothelins
  • Endothelium
  • Gene expression
  • Tissue distribution
  • Vasopressins

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Imai, T., Hirata, Y., Emori, T., Yanagisawa, M., Masaki, T., & Marumo, F. (1992). Induction of endothelin-1 gene by angiotensin and vasopressin in endothelial cells. Hypertension, 19(6 PART 2), 753-757.

Induction of endothelin-1 gene by angiotensin and vasopressin in endothelial cells. / Imai, Taihei; Hirata, Yukio; Emori, Toshiaki; Yanagisawa, Masashi; Masaki, Tomoh; Marumo, Fumiaki.

In: Hypertension, Vol. 19, No. 6 PART 2, 06.1992, p. 753-757.

Research output: Contribution to journalArticle

Imai, T, Hirata, Y, Emori, T, Yanagisawa, M, Masaki, T & Marumo, F 1992, 'Induction of endothelin-1 gene by angiotensin and vasopressin in endothelial cells', Hypertension, vol. 19, no. 6 PART 2, pp. 753-757.
Imai T, Hirata Y, Emori T, Yanagisawa M, Masaki T, Marumo F. Induction of endothelin-1 gene by angiotensin and vasopressin in endothelial cells. Hypertension. 1992 Jun;19(6 PART 2):753-757.
Imai, Taihei ; Hirata, Yukio ; Emori, Toshiaki ; Yanagisawa, Masashi ; Masaki, Tomoh ; Marumo, Fumiaki. / Induction of endothelin-1 gene by angiotensin and vasopressin in endothelial cells. In: Hypertension. 1992 ; Vol. 19, No. 6 PART 2. pp. 753-757.
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