Induction of epithelial-mesenchymal transition in alveolar epithelial cells by transforming growth factor-β1: Potential role in idiopathic pulmonary fibrosis

Brigham C. Willis, Janice M. Liebler, Katherine Luby-Phelps, Andrew G. Nicholson, Edward D. Crandall, Roland M. Du Bois, Zea Borok

Research output: Contribution to journalArticle

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Abstract

The hallmark of idiopathic pulmonary fibrosis (IPF) is the myofibroblast, the cellular origin of which in the lung is unknown. We hypothesized that alveolar epithelial cells (AECs) may serve as a source of myofibroblasts through epithelial-mesenchymal transition (EMT). Effects of chronic exposure to transforming growth factor (TGF)-β1 on the phenotype of isolated rat AECs in primary culture and a rat type II cell line (RLE-6TN) were evaluated. Additionally, tissue samples from patients with IPF were evaluated for cells co-expressing epithelial (thyroid transcription factor (TTF)-1 and pro-surfactant protein-B (pro-SP-B), and mesenchymal (α-smooth muscle actin (α-SMA)) markers. RLE-6TN cells exposed to TGF-β1 for 6 days demonstrated increased expression of mesenchymal cell markers and a fibroblast-like morphology, an effect augmented by tumor necrosis factor-α (TNF-α). Exposure of rat AECs to TGF-β1 (100 pmol/L) resulted in increased expression of α-SMA, type I collagen, vimentin, and desmin, with concurrent transition to a fibroblast-like morphology and decreased expression of TTF-1, aquaporin-5 (AQP5), zonula occludens-1 (ZO-1), and cytokeratins. Cells coexpressing epithelial markers and α-SMA were abundant in lung tissue from IPF patients. These results suggest that AECs undergo EMT when chronically exposed to TGF-β1, raising the possibility that epithelial cells may serve as a novel source of myofibroblasts in IPF.

Original languageEnglish (US)
Pages (from-to)1321-1332
Number of pages12
JournalAmerican Journal of Pathology
Volume166
Issue number5
StatePublished - May 2005

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Alveolar Epithelial Cells
Idiopathic Pulmonary Fibrosis
Epithelial-Mesenchymal Transition
Transforming Growth Factors
Myofibroblasts
Aquaporin 5
Fibroblasts
Epithelial Cells
Lung
Desmin
Tight Junctions
Vimentin
Keratins
Collagen Type I
Surface-Active Agents
Smooth Muscle
Actins
Tumor Necrosis Factor-alpha
Phenotype
Cell Line

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Induction of epithelial-mesenchymal transition in alveolar epithelial cells by transforming growth factor-β1 : Potential role in idiopathic pulmonary fibrosis. / Willis, Brigham C.; Liebler, Janice M.; Luby-Phelps, Katherine; Nicholson, Andrew G.; Crandall, Edward D.; Du Bois, Roland M.; Borok, Zea.

In: American Journal of Pathology, Vol. 166, No. 5, 05.2005, p. 1321-1332.

Research output: Contribution to journalArticle

Willis, Brigham C. ; Liebler, Janice M. ; Luby-Phelps, Katherine ; Nicholson, Andrew G. ; Crandall, Edward D. ; Du Bois, Roland M. ; Borok, Zea. / Induction of epithelial-mesenchymal transition in alveolar epithelial cells by transforming growth factor-β1 : Potential role in idiopathic pulmonary fibrosis. In: American Journal of Pathology. 2005 ; Vol. 166, No. 5. pp. 1321-1332.
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