Induction of murine B cell proliferation by insolubilized anti-immunoglobulins

E. Pure, E. Vitetta

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

This study demonstrates that intact antibodies specific for μ, δ, or light chains when coupled to Sepharose are effective in delivering proliferative signals to B cells. Furthermore, using Sepharose-coupled anti-δ antibodies, we have shown that hybridoma anti-δ (that is not active as soluble protein) is as effective as rabbit anti-δ in activating murine B cells. However, antibodies directed against two other surface molecules, I-A and H-2K, were not mitogenic. Thus, sIgM and sIgD are comparable in their ability to transmit a proliferative signal to the B cell and sIg seems to play a unique role in this regard.

Original languageEnglish (US)
Pages (from-to)1240-1242
Number of pages3
JournalJournal of Immunology
Volume125
Issue number3
StatePublished - 1980

Fingerprint

Immunoglobulins
B-Lymphocytes
Cell Proliferation
Sepharose
Antibodies
Hybridomas
Anti-Idiotypic Antibodies
Rabbits
Light
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Induction of murine B cell proliferation by insolubilized anti-immunoglobulins. / Pure, E.; Vitetta, E.

In: Journal of Immunology, Vol. 125, No. 3, 1980, p. 1240-1242.

Research output: Contribution to journalArticle

@article{e4ff7ec5046f487db298b123548c9ed5,
title = "Induction of murine B cell proliferation by insolubilized anti-immunoglobulins",
abstract = "This study demonstrates that intact antibodies specific for μ, δ, or light chains when coupled to Sepharose are effective in delivering proliferative signals to B cells. Furthermore, using Sepharose-coupled anti-δ antibodies, we have shown that hybridoma anti-δ (that is not active as soluble protein) is as effective as rabbit anti-δ in activating murine B cells. However, antibodies directed against two other surface molecules, I-A and H-2K, were not mitogenic. Thus, sIgM and sIgD are comparable in their ability to transmit a proliferative signal to the B cell and sIg seems to play a unique role in this regard.",
author = "E. Pure and E. Vitetta",
year = "1980",
language = "English (US)",
volume = "125",
pages = "1240--1242",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

}

TY - JOUR

T1 - Induction of murine B cell proliferation by insolubilized anti-immunoglobulins

AU - Pure, E.

AU - Vitetta, E.

PY - 1980

Y1 - 1980

N2 - This study demonstrates that intact antibodies specific for μ, δ, or light chains when coupled to Sepharose are effective in delivering proliferative signals to B cells. Furthermore, using Sepharose-coupled anti-δ antibodies, we have shown that hybridoma anti-δ (that is not active as soluble protein) is as effective as rabbit anti-δ in activating murine B cells. However, antibodies directed against two other surface molecules, I-A and H-2K, were not mitogenic. Thus, sIgM and sIgD are comparable in their ability to transmit a proliferative signal to the B cell and sIg seems to play a unique role in this regard.

AB - This study demonstrates that intact antibodies specific for μ, δ, or light chains when coupled to Sepharose are effective in delivering proliferative signals to B cells. Furthermore, using Sepharose-coupled anti-δ antibodies, we have shown that hybridoma anti-δ (that is not active as soluble protein) is as effective as rabbit anti-δ in activating murine B cells. However, antibodies directed against two other surface molecules, I-A and H-2K, were not mitogenic. Thus, sIgM and sIgD are comparable in their ability to transmit a proliferative signal to the B cell and sIg seems to play a unique role in this regard.

UR - http://www.scopus.com/inward/record.url?scp=0018896890&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018896890&partnerID=8YFLogxK

M3 - Article

C2 - 6967895

AN - SCOPUS:0018896890

VL - 125

SP - 1240

EP - 1242

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 3

ER -