Induction of mutant dynamin specifically blocks endocytic coated vesicle formation

Hanna Damke, Takeshi Baba, Dale E. Warnock, Sandra L. Schmid

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Abstract

Dynamin is the mammalian homologue to the Drosophila shibire gene product. Mutations in this 100-kD GTPase cause a pleiotropic defect in endocytosis. To further investigate its role, we generated stable HeLa cell lines expressing either wild-type dynamin or a mutant defective in GTP binding and hydrolysis driven by a tightly controlled tetracycline-inducible promoter. Overexpression of wild-type dynamin had no effect. In contrast, coated pits failed to become constricted and coated vesicles failed to bud in cells overexpressing mutant dynamin so that endocytosis via both transferrin (Tfn) and EGF receptors was potently inhibited. Coated pit assembly, invagination, and the recruitment of receptors into coated pits were unaffected. Other vesicular transport pathways, including Tfn receptor recycling. Tfn receptor biosynthesis, and cathepsin D transport to lysosomes via Golgi-derived coated vesicles, were unaffected. Bulk fluid-phase uptake also continued at the same initial rates as wild type. EM immunolocalization showed that membrane-bound dynamin was specifically associated with clathrin-coated pits on the plasma membrane. Dynamin was also associated with isolated coated vesicles, suggesting that it plays a role in vesicle budding. Like the Drosophila shibire mutant, HeLa cells overexpressing mutant dynamin accumulated long tubules, many of which remained connected to the plasma membrane. We conclude that dynamin is specifically required for endocytic coated vesicle formation, and that its GTP binding and hydrolysis activities are required to form constricted coated pits and, subsequently, for coated vesicle budding.

Original languageEnglish (US)
Pages (from-to)915-934
Number of pages20
JournalJournal of Cell Biology
Volume127
Issue number4
DOIs
StatePublished - Nov 1994

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Coated Vesicles
Dynamins
Transport Vesicles
Transferrin Receptors
Endocytosis
Guanosine Triphosphate
HeLa Cells
Drosophila
Hydrolysis
Cell Membrane
Cathepsin D
Clathrin
GTP Phosphohydrolases
Transferrin
Lysosomes
Tetracycline
Cell Line
Mutation
Membranes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Induction of mutant dynamin specifically blocks endocytic coated vesicle formation. / Damke, Hanna; Baba, Takeshi; Warnock, Dale E.; Schmid, Sandra L.

In: Journal of Cell Biology, Vol. 127, No. 4, 11.1994, p. 915-934.

Research output: Contribution to journalArticle

Damke, Hanna ; Baba, Takeshi ; Warnock, Dale E. ; Schmid, Sandra L. / Induction of mutant dynamin specifically blocks endocytic coated vesicle formation. In: Journal of Cell Biology. 1994 ; Vol. 127, No. 4. pp. 915-934.
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