Induction of nm23 gene expression in human colonic neoplasms and equal expression in colon tumors of high and low metastatic potential

Mitchell Haut, Patricia S. Steeg, James K V Willson, Sanford D. Markowitz

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

Levels of expression of the murine nm23 gene inversely correlate with metastatic potential in several rodent tumor model systems. Expression of the human nm23 homologue also is lower in human breast cancers of high metastatic potential than in breast cancers of low metastatic potential. In the present study, we examined changes in nm23 expression during colon carcinogenesis as found in 18 matched pairs of normal and neoplastic human colon tissues. We found that a 0.8-kiliobase nm23 transcript was expressed in all samples of morphologically normal colon mucosa. In 16 of 18 colon neoplasms, nm23 expression was further increased in the neoplastic, compared with the morphologically normal, colon mucosa from the same individual. Expression of nm23 was elevated over normal mucosa in 3 of 3 polyps, in 2 of 3 nonmetastatic cancers, and in 11 of the 12 cancers that were metastatic at the initial presentation. The levels of nm23 expressed were similar in the 12 metastatic colon neoplasms and in the 6 colon neoplasms of lower clinical stage. In addition, nm23 expression was maintained in culture in each of 12 cell lines initiated from human colon neoplasms and did not differ between lines established from neoplasms of high or low metastatic capability. We concluded that nm23 was expressed in normal colon mucosa. Expression of nm23 increased during early stages of colon carcinogenesis and remained increased in metastatic colon cancer. Therefore, in the colon, tissue-specific events dissociate nm23 expression from loss of tumor metastatic competence.

Original languageEnglish (US)
Pages (from-to)712-716
Number of pages5
JournalJournal of the National Cancer Institute
Volume83
Issue number10
StatePublished - May 15 1991

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Gene expression
Colonic Neoplasms
Gene Expression
Tumors
Tumor
Proof by induction
Colon
Mucous Membrane
Neoplasms
Tissue
Cancer
Carcinogenesis
Breast Cancer
Cell culture
Breast Neoplasms
Genes
Cells
Matched pairs
Polyps
Human

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging

Cite this

Induction of nm23 gene expression in human colonic neoplasms and equal expression in colon tumors of high and low metastatic potential. / Haut, Mitchell; Steeg, Patricia S.; Willson, James K V; Markowitz, Sanford D.

In: Journal of the National Cancer Institute, Vol. 83, No. 10, 15.05.1991, p. 712-716.

Research output: Contribution to journalArticle

Haut, Mitchell ; Steeg, Patricia S. ; Willson, James K V ; Markowitz, Sanford D. / Induction of nm23 gene expression in human colonic neoplasms and equal expression in colon tumors of high and low metastatic potential. In: Journal of the National Cancer Institute. 1991 ; Vol. 83, No. 10. pp. 712-716.
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