Induction of proliferation and differentiation of murine B cells bearing surface Igλ by rat monoclonal antibody to λ chain

K. Yagawa, E. S. Vitetta

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Abstract

Rats were immunized with the purified BCL1-IgMλ3(1) and their spleen cells were fused with Sp2/0 cells. Hybridomas secreting antibodies relative with λ1-, λ2-, and λ3-containing myeloma proteins were subcloned and propagated in spinner culture. The antibodies were tested in a solid-phase radioimmunoassay and by indirect immunofluorescence using either BCL1 cells or normal mouse spleen cells. One of the monoclonal antibodies was purified to 95% homogeneity on DEAE-Sephadex. The purified anti-λ antibody, coupled to Sepharose, was mitogenic for λ-bearing normal B cells and BCL1 cells, and in the presence of a supernatant from Concanavalin-A (Con A) stimulated these cells to secrete IgMλ.

Original languageEnglish (US)
Pages (from-to)169-175
Number of pages7
JournalHybridoma
Volume2
Issue number2
DOIs
StatePublished - Jan 1 1983

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ASJC Scopus subject areas

  • Immunology
  • Genetics

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