Induction of Protective Immunity to Haemophilus ducreyi in the Temperature-Dependent Rabbit Model of Experimental Chancroid

Eric J. Hansen, Sheryl R. Lumbley, James A. Richardson, Bret K. Purcell, Marla K. Stevens, Leslie D. Cope, Jessica Datte, Justin D. Radolf

Research output: Contribution to journalArticle

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Abstract

The temperature-dependent rabbit model for chancroid, a sexually transmitted disease caused by the fastidious Cram-negative bacterium Haemophilus ducreyi, was used to investigate the abilities of previous infection and immunization with an acellular preparation of H. ducreyi to induce protective immunity. In the first set of experiments, animals were infected intradermally with either the 35000 or Cha-1 strains of H. ducreyi and then rechallenged 30 days later with both the homologous and heterologous strains. In animals infected with the 35000 strain, statistically significant protective immunity occurred only against the homologous strain, whereas protection against both homologous and heterologous challenge was obtained in rabbits previously infected with strain Cha-1. In a separate series of experiments, rabbits were immunized with cell envelopes from either strain 35000 or strain Cha-1 and then challenged with both the homologous and heterologous strains. In rabbits immunized with strain 35000 cell envelopes, significant protective immunity was observed only against challenge with the homologous strain. In animals immunized with strain Cha-1 cell envelopes, protection was obtained against both homologous and heterologous challenge. Histopathologic analysis of sites inoculated with strain 35000 (105 CFU) demonstrated that the inflammatory response in control animals was predominantly suppurative (i.e., heterophilic), whereas that of immunized animals was predominantly mononuclear and, at later time points, largely histiocytic. ELISA and Western blot analyses revealed that immunization produced a better humoral immune response than did infection and provided evidence for antigenic cross-reactivity between these two strains. These results provide the experimental basis for continued efforts to identify potential H. ducreyi vaccinogens.

Original languageEnglish (US)
Pages (from-to)184-192
Number of pages9
JournalJournal of Immunology
Volume152
Issue number1
StatePublished - 1994

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Haemophilus ducreyi
Chancroid
Immunity
Theoretical Models
Rabbits
Temperature
Immunization
Cytoprotection
Humoral Immunity
Sexually Transmitted Diseases
Infection
Western Blotting
Enzyme-Linked Immunosorbent Assay
Bacteria

ASJC Scopus subject areas

  • Immunology

Cite this

Induction of Protective Immunity to Haemophilus ducreyi in the Temperature-Dependent Rabbit Model of Experimental Chancroid. / Hansen, Eric J.; Lumbley, Sheryl R.; Richardson, James A.; Purcell, Bret K.; Stevens, Marla K.; Cope, Leslie D.; Datte, Jessica; Radolf, Justin D.

In: Journal of Immunology, Vol. 152, No. 1, 1994, p. 184-192.

Research output: Contribution to journalArticle

Hansen, EJ, Lumbley, SR, Richardson, JA, Purcell, BK, Stevens, MK, Cope, LD, Datte, J & Radolf, JD 1994, 'Induction of Protective Immunity to Haemophilus ducreyi in the Temperature-Dependent Rabbit Model of Experimental Chancroid', Journal of Immunology, vol. 152, no. 1, pp. 184-192.
Hansen, Eric J. ; Lumbley, Sheryl R. ; Richardson, James A. ; Purcell, Bret K. ; Stevens, Marla K. ; Cope, Leslie D. ; Datte, Jessica ; Radolf, Justin D. / Induction of Protective Immunity to Haemophilus ducreyi in the Temperature-Dependent Rabbit Model of Experimental Chancroid. In: Journal of Immunology. 1994 ; Vol. 152, No. 1. pp. 184-192.
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abstract = "The temperature-dependent rabbit model for chancroid, a sexually transmitted disease caused by the fastidious Cram-negative bacterium Haemophilus ducreyi, was used to investigate the abilities of previous infection and immunization with an acellular preparation of H. ducreyi to induce protective immunity. In the first set of experiments, animals were infected intradermally with either the 35000 or Cha-1 strains of H. ducreyi and then rechallenged 30 days later with both the homologous and heterologous strains. In animals infected with the 35000 strain, statistically significant protective immunity occurred only against the homologous strain, whereas protection against both homologous and heterologous challenge was obtained in rabbits previously infected with strain Cha-1. In a separate series of experiments, rabbits were immunized with cell envelopes from either strain 35000 or strain Cha-1 and then challenged with both the homologous and heterologous strains. In rabbits immunized with strain 35000 cell envelopes, significant protective immunity was observed only against challenge with the homologous strain. In animals immunized with strain Cha-1 cell envelopes, protection was obtained against both homologous and heterologous challenge. Histopathologic analysis of sites inoculated with strain 35000 (105 CFU) demonstrated that the inflammatory response in control animals was predominantly suppurative (i.e., heterophilic), whereas that of immunized animals was predominantly mononuclear and, at later time points, largely histiocytic. ELISA and Western blot analyses revealed that immunization produced a better humoral immune response than did infection and provided evidence for antigenic cross-reactivity between these two strains. These results provide the experimental basis for continued efforts to identify potential H. ducreyi vaccinogens.",
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