Induction of structural changes in the bovine papillomavirus type 1 origin of replication by the viral E1 and E2 proteins

T. G. Gillette, M. Lusky, J. A. Borowiec

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Chemical and enzymatic probing techniques were used to examine the interaction of the bovine papilloma-virus type 1 E1 and E2 proteins with the viral origin of replication (ori). E1 was found to generate significant distortions to the structure of ori, as assayed by KMnO4 oxidation of DNA. The primary site of ori distortion was located within and adjacent to the AT- element of the core replicator sequence, although a number of minor structural transitions were also detected. The induction of these structural changes required ATP and appeared to require ATP hydrolysis. E2 was found to decrease the amount of E1 required for ori distortion but did not significantly alter the pattern of structural distortion. In contrast, the presence of E2 resulted in a biphasic mechanism for E1 binding to ori, as assayed by nuclease protection. Under these conditions, E1 bound preferentially to the dyad symmetry region containing the conserved Hpa I site. Higher levels of El were required for binding to the adjacent ori AT- rich region. Thus, these data suggest that E2 can order the stepwise binding of E1 to ori.

Original languageEnglish (US)
Pages (from-to)8846-8850
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number19
DOIs
StatePublished - 1994

Fingerprint

Bovine papillomavirus 1
Replication Origin
Proteins
Adenosine Triphosphate
AT Rich Sequence
Papilloma
Viral Proteins
Hydrolysis
Viruses
DNA

Keywords

  • DNA replication
  • DNA structure
  • DNA-protein interaction
  • potassium permanganate

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Induction of structural changes in the bovine papillomavirus type 1 origin of replication by the viral E1 and E2 proteins. / Gillette, T. G.; Lusky, M.; Borowiec, J. A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 19, 1994, p. 8846-8850.

Research output: Contribution to journalArticle

@article{03c93e56a5794170851b20ba7463820f,
title = "Induction of structural changes in the bovine papillomavirus type 1 origin of replication by the viral E1 and E2 proteins",
abstract = "Chemical and enzymatic probing techniques were used to examine the interaction of the bovine papilloma-virus type 1 E1 and E2 proteins with the viral origin of replication (ori). E1 was found to generate significant distortions to the structure of ori, as assayed by KMnO4 oxidation of DNA. The primary site of ori distortion was located within and adjacent to the AT- element of the core replicator sequence, although a number of minor structural transitions were also detected. The induction of these structural changes required ATP and appeared to require ATP hydrolysis. E2 was found to decrease the amount of E1 required for ori distortion but did not significantly alter the pattern of structural distortion. In contrast, the presence of E2 resulted in a biphasic mechanism for E1 binding to ori, as assayed by nuclease protection. Under these conditions, E1 bound preferentially to the dyad symmetry region containing the conserved Hpa I site. Higher levels of El were required for binding to the adjacent ori AT- rich region. Thus, these data suggest that E2 can order the stepwise binding of E1 to ori.",
keywords = "DNA replication, DNA structure, DNA-protein interaction, potassium permanganate",
author = "Gillette, {T. G.} and M. Lusky and Borowiec, {J. A.}",
year = "1994",
doi = "10.1073/pnas.91.19.8846",
language = "English (US)",
volume = "91",
pages = "8846--8850",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "19",

}

TY - JOUR

T1 - Induction of structural changes in the bovine papillomavirus type 1 origin of replication by the viral E1 and E2 proteins

AU - Gillette, T. G.

AU - Lusky, M.

AU - Borowiec, J. A.

PY - 1994

Y1 - 1994

N2 - Chemical and enzymatic probing techniques were used to examine the interaction of the bovine papilloma-virus type 1 E1 and E2 proteins with the viral origin of replication (ori). E1 was found to generate significant distortions to the structure of ori, as assayed by KMnO4 oxidation of DNA. The primary site of ori distortion was located within and adjacent to the AT- element of the core replicator sequence, although a number of minor structural transitions were also detected. The induction of these structural changes required ATP and appeared to require ATP hydrolysis. E2 was found to decrease the amount of E1 required for ori distortion but did not significantly alter the pattern of structural distortion. In contrast, the presence of E2 resulted in a biphasic mechanism for E1 binding to ori, as assayed by nuclease protection. Under these conditions, E1 bound preferentially to the dyad symmetry region containing the conserved Hpa I site. Higher levels of El were required for binding to the adjacent ori AT- rich region. Thus, these data suggest that E2 can order the stepwise binding of E1 to ori.

AB - Chemical and enzymatic probing techniques were used to examine the interaction of the bovine papilloma-virus type 1 E1 and E2 proteins with the viral origin of replication (ori). E1 was found to generate significant distortions to the structure of ori, as assayed by KMnO4 oxidation of DNA. The primary site of ori distortion was located within and adjacent to the AT- element of the core replicator sequence, although a number of minor structural transitions were also detected. The induction of these structural changes required ATP and appeared to require ATP hydrolysis. E2 was found to decrease the amount of E1 required for ori distortion but did not significantly alter the pattern of structural distortion. In contrast, the presence of E2 resulted in a biphasic mechanism for E1 binding to ori, as assayed by nuclease protection. Under these conditions, E1 bound preferentially to the dyad symmetry region containing the conserved Hpa I site. Higher levels of El were required for binding to the adjacent ori AT- rich region. Thus, these data suggest that E2 can order the stepwise binding of E1 to ori.

KW - DNA replication

KW - DNA structure

KW - DNA-protein interaction

KW - potassium permanganate

UR - http://www.scopus.com/inward/record.url?scp=0028588659&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028588659&partnerID=8YFLogxK

U2 - 10.1073/pnas.91.19.8846

DO - 10.1073/pnas.91.19.8846

M3 - Article

VL - 91

SP - 8846

EP - 8850

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 19

ER -