TY - JOUR
T1 - Induction of suppurative arthritis in rabbits by Haemophilus endotoxin, tumor necrosis factor-α, and interleukin-1β
AU - Sáez-Llorens, Xavier
AU - Jafari, Hamid S.
AU - Olsen, Kurt D.
AU - Nariuchi, Hideo
AU - Hansen, Eric J.
AU - McCracken, George H.
PY - 1991/1/1
Y1 - 1991/1/1
N2 - Because Haemophilus influenzae type b (Hib) is the principal cause of suppurative arthritis in young children and its lipooligosaccharide (LOS) is thought to be the main virulence factor, Hib endotoxin was evaluated for its ability to induce synovial inflammation in rabbits. Also, the role of the cytokines tumor necrosis factor-α (TNF α) and interleukin-1β (IL-1β) in mediating the synovial inflammatory process was studied. Intraarticular inoculation of 2 pg to 20 ng of Hib LOS produced a dose-dependent increase in concentrations of leukocytes and protein in synovial lavage fluid that was significantly modulated by concomitant administration of rabbit TNFα- and rabbit IL-1β-specific antibodies. Inoculation of joints with either 104 IU of rabbit TNFα or 10 ng recombinant rabbit IL-1β induced synovial inflammatory changes similar to those observed after LOS intraarticular challenge. These data provide evidence for the role of Hib LOS in inducing suppurative arthritis and for the critical participation of TNFα and IL-1β in the initial events of the synovial inflammatory response.
AB - Because Haemophilus influenzae type b (Hib) is the principal cause of suppurative arthritis in young children and its lipooligosaccharide (LOS) is thought to be the main virulence factor, Hib endotoxin was evaluated for its ability to induce synovial inflammation in rabbits. Also, the role of the cytokines tumor necrosis factor-α (TNF α) and interleukin-1β (IL-1β) in mediating the synovial inflammatory process was studied. Intraarticular inoculation of 2 pg to 20 ng of Hib LOS produced a dose-dependent increase in concentrations of leukocytes and protein in synovial lavage fluid that was significantly modulated by concomitant administration of rabbit TNFα- and rabbit IL-1β-specific antibodies. Inoculation of joints with either 104 IU of rabbit TNFα or 10 ng recombinant rabbit IL-1β induced synovial inflammatory changes similar to those observed after LOS intraarticular challenge. These data provide evidence for the role of Hib LOS in inducing suppurative arthritis and for the critical participation of TNFα and IL-1β in the initial events of the synovial inflammatory response.
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U2 - 10.1093/infdis/163.6.1267
DO - 10.1093/infdis/163.6.1267
M3 - Article
C2 - 2037791
AN - SCOPUS:0025939322
VL - 163
SP - 1267
EP - 1272
JO - The Journal of infectious diseases
JF - The Journal of infectious diseases
SN - 0022-1899
IS - 6
ER -