Inexpensive low-oxygen incubators

Woodring E. Wright, Jerry W. Shay

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Although the evidence is overwhelming that ambient oxygen is at least somewhat damaging to most normal cells in culture, the expense and effort involved has resulted in few laboratories growing their cells under physiological oxygen conditions. We here describe how to produce, from commercially available plastic wide-mouth jars, very simple gas-tight containers that can be flushed with prepared gas mixtures to produce low-oxygen environments for standard cell culture. This permits any laboratory to easily try the effects of physiological oxygen on their system without the need for dedicated incubators and substantial monetary investments.

Original languageEnglish (US)
Pages (from-to)2088-2090
Number of pages3
JournalNature Protocols
Volume1
Issue number4
DOIs
StatePublished - Nov 2006

Fingerprint

Incubators
Oxygen
Cell Culture Techniques
Gases
Cell culture
Gas mixtures
Plastics
Containers
Mouth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Inexpensive low-oxygen incubators. / Wright, Woodring E.; Shay, Jerry W.

In: Nature Protocols, Vol. 1, No. 4, 11.2006, p. 2088-2090.

Research output: Contribution to journalArticle

Wright, Woodring E. ; Shay, Jerry W. / Inexpensive low-oxygen incubators. In: Nature Protocols. 2006 ; Vol. 1, No. 4. pp. 2088-2090.
@article{f1662f5e63f54c0fb66dd617dd6f129c,
title = "Inexpensive low-oxygen incubators",
abstract = "Although the evidence is overwhelming that ambient oxygen is at least somewhat damaging to most normal cells in culture, the expense and effort involved has resulted in few laboratories growing their cells under physiological oxygen conditions. We here describe how to produce, from commercially available plastic wide-mouth jars, very simple gas-tight containers that can be flushed with prepared gas mixtures to produce low-oxygen environments for standard cell culture. This permits any laboratory to easily try the effects of physiological oxygen on their system without the need for dedicated incubators and substantial monetary investments.",
author = "Wright, {Woodring E.} and Shay, {Jerry W.}",
year = "2006",
month = "11",
doi = "10.1038/nprot.2006.374",
language = "English (US)",
volume = "1",
pages = "2088--2090",
journal = "Nature Protocols",
issn = "1754-2189",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - Inexpensive low-oxygen incubators

AU - Wright, Woodring E.

AU - Shay, Jerry W.

PY - 2006/11

Y1 - 2006/11

N2 - Although the evidence is overwhelming that ambient oxygen is at least somewhat damaging to most normal cells in culture, the expense and effort involved has resulted in few laboratories growing their cells under physiological oxygen conditions. We here describe how to produce, from commercially available plastic wide-mouth jars, very simple gas-tight containers that can be flushed with prepared gas mixtures to produce low-oxygen environments for standard cell culture. This permits any laboratory to easily try the effects of physiological oxygen on their system without the need for dedicated incubators and substantial monetary investments.

AB - Although the evidence is overwhelming that ambient oxygen is at least somewhat damaging to most normal cells in culture, the expense and effort involved has resulted in few laboratories growing their cells under physiological oxygen conditions. We here describe how to produce, from commercially available plastic wide-mouth jars, very simple gas-tight containers that can be flushed with prepared gas mixtures to produce low-oxygen environments for standard cell culture. This permits any laboratory to easily try the effects of physiological oxygen on their system without the need for dedicated incubators and substantial monetary investments.

UR - http://www.scopus.com/inward/record.url?scp=34248672525&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34248672525&partnerID=8YFLogxK

U2 - 10.1038/nprot.2006.374

DO - 10.1038/nprot.2006.374

M3 - Article

C2 - 17487199

AN - SCOPUS:34248672525

VL - 1

SP - 2088

EP - 2090

JO - Nature Protocols

JF - Nature Protocols

SN - 1754-2189

IS - 4

ER -