Inflammatory and Non-inflammatory Breast Cancer: A Potential Role for Detection of Multiple Viral DNAs in Disease Progression

Mohamed El-Shinawi, Hossam Taha Mohamed, Hadeer Hesham Abdel-Fattah, Sherif Abdel Aziz Ibrahim, Medhat S. El-Halawany, M. Akram Nouh, Robert J. Schneider, Mona Mostafa Mohamed

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Background: Inflammatory breast cancer (IBC) is the most lethal form of breast cancer. Multiple viral infections in IBC tissues were found to be associated with disease pathogenesis. Objective: The aim of the present study was to correlate the incidence of viral DNA with breast cancer progression. Materials and Methods: Overall, 135 women diagnosed with breast cancer were enrolled in this study. Using polymerase chain reaction and sequencing assays, we determined the incidence of human papillomavirus types 16 and 18 (HPV-16 and -18), human cytomegalovirus (HCMV), Epstein–Barr virus, human herpes simplex virus type 1 and 2, and human herpes virus type 8 (HHV-8) in breast carcinoma tissue biopsies. We also assessed the expression of the cell proliferation marker Ki-67 by immunohistochemistry in association with the incidence of viral DNA. Results: HCMV and HPV-16 were the most detected viral DNAs in breast carcinoma tissues; however, the frequency of HCMV and HHV-8 DNA were significantly higher in IBC than non-IBC tissues. Moreover, the prevalence of multiple viral DNAs was higher in IBC than non-IBC tissues. The incidence of multiple viral DNAs positively correlates with tumor size and number of metastatic lymph nodes in both non-IBC and IBC patients. The expression of Ki-67 was found to be significantly higher in both non-IBC and IBC tissues in which multiple viral DNAs were detected. Conclusions: The incidence of multiple viral DNAs in IBC tissues was higher compared with non-IBC tissues. The present results suggest the possibility of a functional relationship between the presence of multiple viral DNAs and disease pathogenesis.

Original languageEnglish (US)
Pages (from-to)494-502
Number of pages9
JournalAnnals of Surgical Oncology
Volume23
Issue number2
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Oncology

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    El-Shinawi, M., Mohamed, H. T., Abdel-Fattah, H. H., Ibrahim, S. A. A., El-Halawany, M. S., Nouh, M. A., Schneider, R. J., & Mohamed, M. M. (2016). Inflammatory and Non-inflammatory Breast Cancer: A Potential Role for Detection of Multiple Viral DNAs in Disease Progression. Annals of Surgical Oncology, 23(2), 494-502. https://doi.org/10.1245/s10434-015-4888-2