TY - JOUR
T1 - Inflammatory and Non-inflammatory Breast Cancer
T2 - A Potential Role for Detection of Multiple Viral DNAs in Disease Progression
AU - El-Shinawi, Mohamed
AU - Mohamed, Hossam Taha
AU - Abdel-Fattah, Hadeer Hesham
AU - Ibrahim, Sherif Abdel Aziz
AU - El-Halawany, Medhat S.
AU - Nouh, M. Akram
AU - Schneider, Robert J.
AU - Mohamed, Mona Mostafa
N1 - Funding Information:
This work was conducted at the Cancer Biology Research Laboratory (CBRL), Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt, and was supported by Avon Foundation Grants # 02-2009-085 a and b (Robert J. Schneider and Mona Mostafa Mohamed). A special thanks to Dr. Yasser Hassane, Summit Urgent Care, Macomb, MI, USA, for donating the reagents used in this study.
Publisher Copyright:
© 2015, Society of Surgical Oncology.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Background: Inflammatory breast cancer (IBC) is the most lethal form of breast cancer. Multiple viral infections in IBC tissues were found to be associated with disease pathogenesis. Objective: The aim of the present study was to correlate the incidence of viral DNA with breast cancer progression. Materials and Methods: Overall, 135 women diagnosed with breast cancer were enrolled in this study. Using polymerase chain reaction and sequencing assays, we determined the incidence of human papillomavirus types 16 and 18 (HPV-16 and -18), human cytomegalovirus (HCMV), Epstein–Barr virus, human herpes simplex virus type 1 and 2, and human herpes virus type 8 (HHV-8) in breast carcinoma tissue biopsies. We also assessed the expression of the cell proliferation marker Ki-67 by immunohistochemistry in association with the incidence of viral DNA. Results: HCMV and HPV-16 were the most detected viral DNAs in breast carcinoma tissues; however, the frequency of HCMV and HHV-8 DNA were significantly higher in IBC than non-IBC tissues. Moreover, the prevalence of multiple viral DNAs was higher in IBC than non-IBC tissues. The incidence of multiple viral DNAs positively correlates with tumor size and number of metastatic lymph nodes in both non-IBC and IBC patients. The expression of Ki-67 was found to be significantly higher in both non-IBC and IBC tissues in which multiple viral DNAs were detected. Conclusions: The incidence of multiple viral DNAs in IBC tissues was higher compared with non-IBC tissues. The present results suggest the possibility of a functional relationship between the presence of multiple viral DNAs and disease pathogenesis.
AB - Background: Inflammatory breast cancer (IBC) is the most lethal form of breast cancer. Multiple viral infections in IBC tissues were found to be associated with disease pathogenesis. Objective: The aim of the present study was to correlate the incidence of viral DNA with breast cancer progression. Materials and Methods: Overall, 135 women diagnosed with breast cancer were enrolled in this study. Using polymerase chain reaction and sequencing assays, we determined the incidence of human papillomavirus types 16 and 18 (HPV-16 and -18), human cytomegalovirus (HCMV), Epstein–Barr virus, human herpes simplex virus type 1 and 2, and human herpes virus type 8 (HHV-8) in breast carcinoma tissue biopsies. We also assessed the expression of the cell proliferation marker Ki-67 by immunohistochemistry in association with the incidence of viral DNA. Results: HCMV and HPV-16 were the most detected viral DNAs in breast carcinoma tissues; however, the frequency of HCMV and HHV-8 DNA were significantly higher in IBC than non-IBC tissues. Moreover, the prevalence of multiple viral DNAs was higher in IBC than non-IBC tissues. The incidence of multiple viral DNAs positively correlates with tumor size and number of metastatic lymph nodes in both non-IBC and IBC patients. The expression of Ki-67 was found to be significantly higher in both non-IBC and IBC tissues in which multiple viral DNAs were detected. Conclusions: The incidence of multiple viral DNAs in IBC tissues was higher compared with non-IBC tissues. The present results suggest the possibility of a functional relationship between the presence of multiple viral DNAs and disease pathogenesis.
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U2 - 10.1245/s10434-015-4888-2
DO - 10.1245/s10434-015-4888-2
M3 - Article
C2 - 26508152
AN - SCOPUS:84958159372
SN - 1068-9265
VL - 23
SP - 494
EP - 502
JO - Annals of Surgical Oncology
JF - Annals of Surgical Oncology
IS - 2
ER -