Influence of a fibric acid type of hypolipidemic agent on the oxidative metabolism of arachidonic acid by liver microsomal cytochrome P-450

J. Capdevila, Y. R. Kim, C. Martin-Wixtrom, J R Falck, S. Manna, R. W. Estabrook

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The regiospecificity of arachidonic acid oxygenation, catalyzed by rat liver microsomal fractions in the presence of NADPH, can be altered by animal pretreatment with a fibric acid type of hypolipidemic drug, ciprofibrate. While microsomal fractions isolated from either control or phenobarbital-treated animals oxygenate arachidonic acid to mainly epoxyeicosatrienoic acids (EETs), animal pretreatment with ciprofibrate results in an eightfold stimulation of ω and ω-1 oxidation, concomitant with a net decrease in the formation of both HETEs and EETs. The isomeric composition of the EETs and of the ω and ω-1 oxidation products formed is also dependent on the type of animal pretreatment. Associated decreases in the amounts of HETEs and the rate of hydrogen peroxide formation suggests a modification of the "uncoupler action" of arachidonic acid during the function of different cytochromes P-450.

Original languageEnglish (US)
Pages (from-to)8-19
Number of pages12
JournalArchives of Biochemistry and Biophysics
Volume243
Issue number1
DOIs
StatePublished - Nov 15 1985

Fingerprint

Hypolipidemic Agents
Arachidonic Acid
Metabolism
Liver
Cytochrome P-450 Enzyme System
Animals
Hydroxyeicosatetraenoic Acids
Oxidation
Oxygenation
Phenobarbital
NADP
Hydrogen Peroxide
Rats
Acids
fibric acid
Chemical analysis
ciprofibrate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Influence of a fibric acid type of hypolipidemic agent on the oxidative metabolism of arachidonic acid by liver microsomal cytochrome P-450. / Capdevila, J.; Kim, Y. R.; Martin-Wixtrom, C.; Falck, J R; Manna, S.; Estabrook, R. W.

In: Archives of Biochemistry and Biophysics, Vol. 243, No. 1, 15.11.1985, p. 8-19.

Research output: Contribution to journalArticle

@article{789016b07a6a4790933d451adf6dcc02,
title = "Influence of a fibric acid type of hypolipidemic agent on the oxidative metabolism of arachidonic acid by liver microsomal cytochrome P-450",
abstract = "The regiospecificity of arachidonic acid oxygenation, catalyzed by rat liver microsomal fractions in the presence of NADPH, can be altered by animal pretreatment with a fibric acid type of hypolipidemic drug, ciprofibrate. While microsomal fractions isolated from either control or phenobarbital-treated animals oxygenate arachidonic acid to mainly epoxyeicosatrienoic acids (EETs), animal pretreatment with ciprofibrate results in an eightfold stimulation of ω and ω-1 oxidation, concomitant with a net decrease in the formation of both HETEs and EETs. The isomeric composition of the EETs and of the ω and ω-1 oxidation products formed is also dependent on the type of animal pretreatment. Associated decreases in the amounts of HETEs and the rate of hydrogen peroxide formation suggests a modification of the {"}uncoupler action{"} of arachidonic acid during the function of different cytochromes P-450.",
author = "J. Capdevila and Kim, {Y. R.} and C. Martin-Wixtrom and Falck, {J R} and S. Manna and Estabrook, {R. W.}",
year = "1985",
month = "11",
day = "15",
doi = "10.1016/0003-9861(85)90768-4",
language = "English (US)",
volume = "243",
pages = "8--19",
journal = "Archives of Biochemistry and Biophysics",
issn = "0003-9861",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Influence of a fibric acid type of hypolipidemic agent on the oxidative metabolism of arachidonic acid by liver microsomal cytochrome P-450

AU - Capdevila, J.

AU - Kim, Y. R.

AU - Martin-Wixtrom, C.

AU - Falck, J R

AU - Manna, S.

AU - Estabrook, R. W.

PY - 1985/11/15

Y1 - 1985/11/15

N2 - The regiospecificity of arachidonic acid oxygenation, catalyzed by rat liver microsomal fractions in the presence of NADPH, can be altered by animal pretreatment with a fibric acid type of hypolipidemic drug, ciprofibrate. While microsomal fractions isolated from either control or phenobarbital-treated animals oxygenate arachidonic acid to mainly epoxyeicosatrienoic acids (EETs), animal pretreatment with ciprofibrate results in an eightfold stimulation of ω and ω-1 oxidation, concomitant with a net decrease in the formation of both HETEs and EETs. The isomeric composition of the EETs and of the ω and ω-1 oxidation products formed is also dependent on the type of animal pretreatment. Associated decreases in the amounts of HETEs and the rate of hydrogen peroxide formation suggests a modification of the "uncoupler action" of arachidonic acid during the function of different cytochromes P-450.

AB - The regiospecificity of arachidonic acid oxygenation, catalyzed by rat liver microsomal fractions in the presence of NADPH, can be altered by animal pretreatment with a fibric acid type of hypolipidemic drug, ciprofibrate. While microsomal fractions isolated from either control or phenobarbital-treated animals oxygenate arachidonic acid to mainly epoxyeicosatrienoic acids (EETs), animal pretreatment with ciprofibrate results in an eightfold stimulation of ω and ω-1 oxidation, concomitant with a net decrease in the formation of both HETEs and EETs. The isomeric composition of the EETs and of the ω and ω-1 oxidation products formed is also dependent on the type of animal pretreatment. Associated decreases in the amounts of HETEs and the rate of hydrogen peroxide formation suggests a modification of the "uncoupler action" of arachidonic acid during the function of different cytochromes P-450.

UR - http://www.scopus.com/inward/record.url?scp=0022366610&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022366610&partnerID=8YFLogxK

U2 - 10.1016/0003-9861(85)90768-4

DO - 10.1016/0003-9861(85)90768-4

M3 - Article

VL - 243

SP - 8

EP - 19

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - 1

ER -