Influence of cardiopulmonary bypass on platelet and neutrophil accumulations in internal organs

Rohan Bhujle, Jun Li, Parag Shastri, John N. Gaffke, James E. Clift, Yun Wei Ye, Michael L. Dollar, Peter Ching, Robert Chao, Anca Constantinescu, Padmakar V Kulkarni, Qin Chang Cheng, Frank Wians, Michael E Jessen, Robert C. Eberhart

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The authors employed gamma scintigraphy to quantify the post bypass accumulations of platelets and neutrophils in the lung, liver, and heart of adult pigs subjected to a standard 90 min regimen of normothermic cardiopulmonary bypass (CPB). Coated and uncoated microporous polypropylene oxygenator circuits were studied for Cobe Duo (Arvada, CO) oxygenators (amphophilic silicone-caprolactone oligomer [SMA] coating, n = 8 each) and Medtronic Maxima (Irvine, CA) oxygenators (Carmeda heparin coating, n = 5 each). Images of cells in the organs (deposited + blood pool) were corrected for tissue absorption and other factors and compared for a 2 hr period post CPB, using repeat measures ANOVA and rank tests. Platelet accumulations in internal organs correlated positively with whole blood platelet counts and negatively with platelet deposits in oxygenators during CPB. In general, uncoated CPB circuits significantly reduced platelet and neutrophil accumulations in lung, liver, and heart versus preCPB controls for the post CPB interval, for both systems. The SMA treatment significantly increased platelet accumulations versus uncoated controls in lung, liver, and heart for the 2 hr period, including the majority of the post CPB sampling intervals; platelet densities did not reach preCPB levels. Neutrophil accumulations were unaffected by the SMA coating. Carmeda heparin treatment significantly increased platelet accumulations in the liver, but not lung or heart. Despite preservation of circulating neutrophils observed with the Carmeda heparin treatment, neutrophil accumulations in internal organs were not elevated post CPB.

Original languageEnglish (US)
JournalASAIO Journal
Volume43
Issue number5
StatePublished - Sep 1997

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Platelets
Cardiopulmonary Bypass
Neutrophils
Blood Platelets
Oxygenators
Liver
Heparin
Lung
Coatings
Blood
Polypropylenes
Silicones
Carbon Monoxide
Networks (circuits)
Platelet Count
Radionuclide Imaging
Analysis of variance (ANOVA)
Oligomers
Analysis of Variance
Swine

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering

Cite this

Bhujle, R., Li, J., Shastri, P., Gaffke, J. N., Clift, J. E., Ye, Y. W., ... Eberhart, R. C. (1997). Influence of cardiopulmonary bypass on platelet and neutrophil accumulations in internal organs. ASAIO Journal, 43(5).

Influence of cardiopulmonary bypass on platelet and neutrophil accumulations in internal organs. / Bhujle, Rohan; Li, Jun; Shastri, Parag; Gaffke, John N.; Clift, James E.; Ye, Yun Wei; Dollar, Michael L.; Ching, Peter; Chao, Robert; Constantinescu, Anca; Kulkarni, Padmakar V; Cheng, Qin Chang; Wians, Frank; Jessen, Michael E; Eberhart, Robert C.

In: ASAIO Journal, Vol. 43, No. 5, 09.1997.

Research output: Contribution to journalArticle

Bhujle, R, Li, J, Shastri, P, Gaffke, JN, Clift, JE, Ye, YW, Dollar, ML, Ching, P, Chao, R, Constantinescu, A, Kulkarni, PV, Cheng, QC, Wians, F, Jessen, ME & Eberhart, RC 1997, 'Influence of cardiopulmonary bypass on platelet and neutrophil accumulations in internal organs', ASAIO Journal, vol. 43, no. 5.
Bhujle R, Li J, Shastri P, Gaffke JN, Clift JE, Ye YW et al. Influence of cardiopulmonary bypass on platelet and neutrophil accumulations in internal organs. ASAIO Journal. 1997 Sep;43(5).
Bhujle, Rohan ; Li, Jun ; Shastri, Parag ; Gaffke, John N. ; Clift, James E. ; Ye, Yun Wei ; Dollar, Michael L. ; Ching, Peter ; Chao, Robert ; Constantinescu, Anca ; Kulkarni, Padmakar V ; Cheng, Qin Chang ; Wians, Frank ; Jessen, Michael E ; Eberhart, Robert C. / Influence of cardiopulmonary bypass on platelet and neutrophil accumulations in internal organs. In: ASAIO Journal. 1997 ; Vol. 43, No. 5.
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