Influence of DNA repair deficiencies on the UV sensitivity of yeast cells in different cell cycle stages

Wolfram Siede; Errol C. Friedberg

doi:10.1016/0165-7992(90)90158-G

Influence of DNA repair deficiencies on the UV sensitivity of yeast cells in different cell cycle stages

Wolfram Siede, Errol C. Friedberg

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26 Scopus citations

Abstract

Synchronously dividing haploid yeast cells were UV-irradiated in various stages of the cell cycle after release from α-factor arrest. In confirmation of earlier results (Chanet et al., 1973), in wild-type strains G1/S phase cells were found to be the most sensitive and late S/G2 cells the most resistant. Stationary-phase (G₀) cells were significantly more UV resistant than G1 cells. Strains defective in nucleotide excision repair lost enhanced resistance in the G2 phase and were most UV-sensitive in the G_o state. Reduced G2 resistance was also observed in rad6 mutants but not in rad9 mutants. After UV-irradiation in G1 phase rad9 mutant cells showed a reduced G1/S phase arrest.

Original language	English (US)
Pages (from-to)	287-292
Number of pages	6
Journal	Mutation Research Letters
Volume	245
Issue number	4
DOIs	https://doi.org/10.1016/0165-7992(90)90158-G
State	Published - Dec 1990

Keywords

Cell cycle
DNA-repair deficiencies
Excision repair
Rad genes
UV sensitivity
Yeast

ASJC Scopus subject areas

General Medicine

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10.1016/0165-7992(90)90158-G

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title = "Influence of DNA repair deficiencies on the UV sensitivity of yeast cells in different cell cycle stages",

abstract = "Synchronously dividing haploid yeast cells were UV-irradiated in various stages of the cell cycle after release from α-factor arrest. In confirmation of earlier results (Chanet et al., 1973), in wild-type strains G1/S phase cells were found to be the most sensitive and late S/G2 cells the most resistant. Stationary-phase (G0) cells were significantly more UV resistant than G1 cells. Strains defective in nucleotide excision repair lost enhanced resistance in the G2 phase and were most UV-sensitive in the Go state. Reduced G2 resistance was also observed in rad6 mutants but not in rad9 mutants. After UV-irradiation in G1 phase rad9 mutant cells showed a reduced G1/S phase arrest.",

keywords = "Cell cycle, DNA-repair deficiencies, Excision repair, Rad genes, UV sensitivity, Yeast",

author = "Wolfram Siede and Friedberg, {Errol C.}",

note = "Funding Information: These studies were supported by research grant CA-12428 from the United States Publish Health Service.",

year = "1990",

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language = "English (US)",

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T1 - Influence of DNA repair deficiencies on the UV sensitivity of yeast cells in different cell cycle stages

AU - Siede, Wolfram

AU - Friedberg, Errol C.

N1 - Funding Information: These studies were supported by research grant CA-12428 from the United States Publish Health Service.

PY - 1990/12

Y1 - 1990/12

N2 - Synchronously dividing haploid yeast cells were UV-irradiated in various stages of the cell cycle after release from α-factor arrest. In confirmation of earlier results (Chanet et al., 1973), in wild-type strains G1/S phase cells were found to be the most sensitive and late S/G2 cells the most resistant. Stationary-phase (G0) cells were significantly more UV resistant than G1 cells. Strains defective in nucleotide excision repair lost enhanced resistance in the G2 phase and were most UV-sensitive in the Go state. Reduced G2 resistance was also observed in rad6 mutants but not in rad9 mutants. After UV-irradiation in G1 phase rad9 mutant cells showed a reduced G1/S phase arrest.

AB - Synchronously dividing haploid yeast cells were UV-irradiated in various stages of the cell cycle after release from α-factor arrest. In confirmation of earlier results (Chanet et al., 1973), in wild-type strains G1/S phase cells were found to be the most sensitive and late S/G2 cells the most resistant. Stationary-phase (G0) cells were significantly more UV resistant than G1 cells. Strains defective in nucleotide excision repair lost enhanced resistance in the G2 phase and were most UV-sensitive in the Go state. Reduced G2 resistance was also observed in rad6 mutants but not in rad9 mutants. After UV-irradiation in G1 phase rad9 mutant cells showed a reduced G1/S phase arrest.

KW - Cell cycle

KW - DNA-repair deficiencies

KW - Excision repair

KW - Rad genes

KW - UV sensitivity

KW - Yeast

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Influence of DNA repair deficiencies on the UV sensitivity of yeast cells in different cell cycle stages

Abstract

Keywords

ASJC Scopus subject areas

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