Influence of DNA repair deficiencies on the UV sensitivity of yeast cells in different cell cycle stages

Wolfram Siede, Errol C. Friedberg

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Synchronously dividing haploid yeast cells were UV-irradiated in various stages of the cell cycle after release from α-factor arrest. In confirmation of earlier results (Chanet et al., 1973), in wild-type strains G1/S phase cells were found to be the most sensitive and late S/G2 cells the most resistant. Stationary-phase (G0) cells were significantly more UV resistant than G1 cells. Strains defective in nucleotide excision repair lost enhanced resistance in the G2 phase and were most UV-sensitive in the Go state. Reduced G2 resistance was also observed in rad6 mutants but not in rad9 mutants. After UV-irradiation in G1 phase rad9 mutant cells showed a reduced G1/S phase arrest.

Original languageEnglish (US)
Pages (from-to)287-292
Number of pages6
JournalMutation Research Letters
Volume245
Issue number4
DOIs
StatePublished - Dec 1990

Keywords

  • Cell cycle
  • DNA-repair deficiencies
  • Excision repair
  • Rad genes
  • UV sensitivity
  • Yeast

ASJC Scopus subject areas

  • Medicine(all)

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