Influence of estradiol administration on protein synthesis in homogenates of hen oviduct

Jean D. Wilson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Recent studies in this laboratory on protein synthesis in slices of seminal vesicle have demonstrated that the administration of testosterone to immature rats results in the enhancement of a specific step in protein synthesis, the peptide bonding of transfer RNA-amino acids to form microsomal ribonucleo-protein (Wilson, 1962). However, the formation of peptide bonds in protein biosynthesis is a complex reaction, requiring, in addition to the transfer RNA-amino acids and the ribosome acceptor, the cofactor GTP, a soluble transfer enzyme, magnesium, and, in some preparations, sulfhydryl compounds (Nathans and Lipmann, 1961). In order, therefore, to localize further the mechanism of this hormonal action, the influence of estradiol administration on protein synthesis has been studied in cell-free preparations of immature hen oviduct. The hen oviduct was chosen for this study because of the demonstration by Brandt and Nalbandov (1956) that protein synthesis in this tissue is regulated by both androgens and estrogens.

Original languageEnglish (US)
Pages (from-to)175-179
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume8
Issue number3
StatePublished - Jul 3 1962

Fingerprint

Oviducts
Estradiol
Transfer RNA
Proteins
Amino Acids
Peptides
Seminal Vesicles
Protein Biosynthesis
Guanosine Triphosphate
Ribosomes
Sulfhydryl Compounds
Biosynthesis
Magnesium
Androgens
Testosterone
Estrogens
Rats
Demonstrations
Tissue
Enzymes

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Influence of estradiol administration on protein synthesis in homogenates of hen oviduct. / Wilson, Jean D.

In: Biochemical and Biophysical Research Communications, Vol. 8, No. 3, 03.07.1962, p. 175-179.

Research output: Contribution to journalArticle

@article{6fb63ed830ea45a18b6883b70d49ae18,
title = "Influence of estradiol administration on protein synthesis in homogenates of hen oviduct",
abstract = "Recent studies in this laboratory on protein synthesis in slices of seminal vesicle have demonstrated that the administration of testosterone to immature rats results in the enhancement of a specific step in protein synthesis, the peptide bonding of transfer RNA-amino acids to form microsomal ribonucleo-protein (Wilson, 1962). However, the formation of peptide bonds in protein biosynthesis is a complex reaction, requiring, in addition to the transfer RNA-amino acids and the ribosome acceptor, the cofactor GTP, a soluble transfer enzyme, magnesium, and, in some preparations, sulfhydryl compounds (Nathans and Lipmann, 1961). In order, therefore, to localize further the mechanism of this hormonal action, the influence of estradiol administration on protein synthesis has been studied in cell-free preparations of immature hen oviduct. The hen oviduct was chosen for this study because of the demonstration by Brandt and Nalbandov (1956) that protein synthesis in this tissue is regulated by both androgens and estrogens.",
author = "Wilson, {Jean D.}",
year = "1962",
month = "7",
day = "3",
language = "English (US)",
volume = "8",
pages = "175--179",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Influence of estradiol administration on protein synthesis in homogenates of hen oviduct

AU - Wilson, Jean D.

PY - 1962/7/3

Y1 - 1962/7/3

N2 - Recent studies in this laboratory on protein synthesis in slices of seminal vesicle have demonstrated that the administration of testosterone to immature rats results in the enhancement of a specific step in protein synthesis, the peptide bonding of transfer RNA-amino acids to form microsomal ribonucleo-protein (Wilson, 1962). However, the formation of peptide bonds in protein biosynthesis is a complex reaction, requiring, in addition to the transfer RNA-amino acids and the ribosome acceptor, the cofactor GTP, a soluble transfer enzyme, magnesium, and, in some preparations, sulfhydryl compounds (Nathans and Lipmann, 1961). In order, therefore, to localize further the mechanism of this hormonal action, the influence of estradiol administration on protein synthesis has been studied in cell-free preparations of immature hen oviduct. The hen oviduct was chosen for this study because of the demonstration by Brandt and Nalbandov (1956) that protein synthesis in this tissue is regulated by both androgens and estrogens.

AB - Recent studies in this laboratory on protein synthesis in slices of seminal vesicle have demonstrated that the administration of testosterone to immature rats results in the enhancement of a specific step in protein synthesis, the peptide bonding of transfer RNA-amino acids to form microsomal ribonucleo-protein (Wilson, 1962). However, the formation of peptide bonds in protein biosynthesis is a complex reaction, requiring, in addition to the transfer RNA-amino acids and the ribosome acceptor, the cofactor GTP, a soluble transfer enzyme, magnesium, and, in some preparations, sulfhydryl compounds (Nathans and Lipmann, 1961). In order, therefore, to localize further the mechanism of this hormonal action, the influence of estradiol administration on protein synthesis has been studied in cell-free preparations of immature hen oviduct. The hen oviduct was chosen for this study because of the demonstration by Brandt and Nalbandov (1956) that protein synthesis in this tissue is regulated by both androgens and estrogens.

UR - http://www.scopus.com/inward/record.url?scp=50549182267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50549182267&partnerID=8YFLogxK

M3 - Article

C2 - 14007368

AN - SCOPUS:50549182267

VL - 8

SP - 175

EP - 179

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -