Influence of the major nitrite transporter NirC on the virulence of a Swollen Head Syndrome Avian Pathogenic E. coli (APEC) strain

Jacqueline Boldrin de Paiva, Janaína Luisa Leite, Livia Pilatti Mendes da Silva, Thais Cabrera Galvão Rojas, Fernanda de Pace, Rogério Arcuri Conceição, Vanessa Sperandio, Wanderley Dias da Silveira

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Avian Pathogenic Escherichia coli (APEC) strains are extra-intestinal E. coli that infect poultry and cause diseases. Nitrite is a central branch-point in bacterial nitrogen metabolism and is used as a cytotoxin by macrophages. Unlike nitric oxide (NO), nitrite cannot diffuse across bacterial membrane cells. The NirC protein acts as a specific channel to facilitate the transport of nitrite into Salmonella and E. coli cells for nitrogen metabolism and cytoplasmic detoxification. NirC is also required for the pathogenicity of Salmonella by downregulating the production of NO by the host macrophages. Based on an in vitro microarray that revealed the overexpression of the nirC gene in APEC strain SCI-07, we constructed a nirC-deficient SCI-07 strain (δ. nirC) and evaluated its virulence potential using in vivo and in vitro assays. The final cumulative mortalities caused by mutant and wild-type (WT) were similar; while the δ. nirC caused a gradual increase in the mortality rate during the seven days recorded, the WT caused mortality up to 24. h post-infection (hpi). Counts of the δ. nirC cells in the spleen, lung and liver were higher than those of the WT after 48. hpi but similar at 24. hpi. Although similar number of δ. nirC and WT cells was observed in macrophages at 3. hpi, there was higher number of δ. nirC cells at 16. hpi. The cell adhesion ability of the δ. nirC strain was about half the WT level in the presence and absence of alpha-. d-mannopyranoside. These results indicate that the nirC gene influences the pathogenicity of SCI-07 strain.

Original languageEnglish (US)
Pages (from-to)123-131
Number of pages9
JournalVeterinary Microbiology
Volume175
Issue number1
DOIs
StatePublished - Jan 30 2015

Fingerprint

Nitrites
nitrites
Virulence
transporters
virulence
Head
Escherichia coli
Infection
macrophages
infection
Macrophages
nitrogen metabolism
Salmonella
nitric oxide
Mortality
Nitric Oxide
Nitrogen
pathogenicity
Poultry Diseases
cytotoxins

Keywords

  • APEC
  • Escherichia coli
  • NirC
  • Pathogenicity
  • Virulence

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

de Paiva, J. B., Leite, J. L., da Silva, L. P. M., Rojas, T. C. G., de Pace, F., Conceição, R. A., ... da Silveira, W. D. (2015). Influence of the major nitrite transporter NirC on the virulence of a Swollen Head Syndrome Avian Pathogenic E. coli (APEC) strain. Veterinary Microbiology, 175(1), 123-131. https://doi.org/10.1016/j.vetmic.2014.11.015

Influence of the major nitrite transporter NirC on the virulence of a Swollen Head Syndrome Avian Pathogenic E. coli (APEC) strain. / de Paiva, Jacqueline Boldrin; Leite, Janaína Luisa; da Silva, Livia Pilatti Mendes; Rojas, Thais Cabrera Galvão; de Pace, Fernanda; Conceição, Rogério Arcuri; Sperandio, Vanessa; da Silveira, Wanderley Dias.

In: Veterinary Microbiology, Vol. 175, No. 1, 30.01.2015, p. 123-131.

Research output: Contribution to journalArticle

de Paiva, Jacqueline Boldrin ; Leite, Janaína Luisa ; da Silva, Livia Pilatti Mendes ; Rojas, Thais Cabrera Galvão ; de Pace, Fernanda ; Conceição, Rogério Arcuri ; Sperandio, Vanessa ; da Silveira, Wanderley Dias. / Influence of the major nitrite transporter NirC on the virulence of a Swollen Head Syndrome Avian Pathogenic E. coli (APEC) strain. In: Veterinary Microbiology. 2015 ; Vol. 175, No. 1. pp. 123-131.
@article{3ae104e0743c4f7a893682d1b0a8acbb,
title = "Influence of the major nitrite transporter NirC on the virulence of a Swollen Head Syndrome Avian Pathogenic E. coli (APEC) strain",
abstract = "Avian Pathogenic Escherichia coli (APEC) strains are extra-intestinal E. coli that infect poultry and cause diseases. Nitrite is a central branch-point in bacterial nitrogen metabolism and is used as a cytotoxin by macrophages. Unlike nitric oxide (NO), nitrite cannot diffuse across bacterial membrane cells. The NirC protein acts as a specific channel to facilitate the transport of nitrite into Salmonella and E. coli cells for nitrogen metabolism and cytoplasmic detoxification. NirC is also required for the pathogenicity of Salmonella by downregulating the production of NO by the host macrophages. Based on an in vitro microarray that revealed the overexpression of the nirC gene in APEC strain SCI-07, we constructed a nirC-deficient SCI-07 strain (δ. nirC) and evaluated its virulence potential using in vivo and in vitro assays. The final cumulative mortalities caused by mutant and wild-type (WT) were similar; while the δ. nirC caused a gradual increase in the mortality rate during the seven days recorded, the WT caused mortality up to 24. h post-infection (hpi). Counts of the δ. nirC cells in the spleen, lung and liver were higher than those of the WT after 48. hpi but similar at 24. hpi. Although similar number of δ. nirC and WT cells was observed in macrophages at 3. hpi, there was higher number of δ. nirC cells at 16. hpi. The cell adhesion ability of the δ. nirC strain was about half the WT level in the presence and absence of alpha-. d-mannopyranoside. These results indicate that the nirC gene influences the pathogenicity of SCI-07 strain.",
keywords = "APEC, Escherichia coli, NirC, Pathogenicity, Virulence",
author = "{de Paiva}, {Jacqueline Boldrin} and Leite, {Jana{\'i}na Luisa} and {da Silva}, {Livia Pilatti Mendes} and Rojas, {Thais Cabrera Galv{\~a}o} and {de Pace}, Fernanda and Concei{\cc}{\~a}o, {Rog{\'e}rio Arcuri} and Vanessa Sperandio and {da Silveira}, {Wanderley Dias}",
year = "2015",
month = "1",
day = "30",
doi = "10.1016/j.vetmic.2014.11.015",
language = "English (US)",
volume = "175",
pages = "123--131",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Influence of the major nitrite transporter NirC on the virulence of a Swollen Head Syndrome Avian Pathogenic E. coli (APEC) strain

AU - de Paiva, Jacqueline Boldrin

AU - Leite, Janaína Luisa

AU - da Silva, Livia Pilatti Mendes

AU - Rojas, Thais Cabrera Galvão

AU - de Pace, Fernanda

AU - Conceição, Rogério Arcuri

AU - Sperandio, Vanessa

AU - da Silveira, Wanderley Dias

PY - 2015/1/30

Y1 - 2015/1/30

N2 - Avian Pathogenic Escherichia coli (APEC) strains are extra-intestinal E. coli that infect poultry and cause diseases. Nitrite is a central branch-point in bacterial nitrogen metabolism and is used as a cytotoxin by macrophages. Unlike nitric oxide (NO), nitrite cannot diffuse across bacterial membrane cells. The NirC protein acts as a specific channel to facilitate the transport of nitrite into Salmonella and E. coli cells for nitrogen metabolism and cytoplasmic detoxification. NirC is also required for the pathogenicity of Salmonella by downregulating the production of NO by the host macrophages. Based on an in vitro microarray that revealed the overexpression of the nirC gene in APEC strain SCI-07, we constructed a nirC-deficient SCI-07 strain (δ. nirC) and evaluated its virulence potential using in vivo and in vitro assays. The final cumulative mortalities caused by mutant and wild-type (WT) were similar; while the δ. nirC caused a gradual increase in the mortality rate during the seven days recorded, the WT caused mortality up to 24. h post-infection (hpi). Counts of the δ. nirC cells in the spleen, lung and liver were higher than those of the WT after 48. hpi but similar at 24. hpi. Although similar number of δ. nirC and WT cells was observed in macrophages at 3. hpi, there was higher number of δ. nirC cells at 16. hpi. The cell adhesion ability of the δ. nirC strain was about half the WT level in the presence and absence of alpha-. d-mannopyranoside. These results indicate that the nirC gene influences the pathogenicity of SCI-07 strain.

AB - Avian Pathogenic Escherichia coli (APEC) strains are extra-intestinal E. coli that infect poultry and cause diseases. Nitrite is a central branch-point in bacterial nitrogen metabolism and is used as a cytotoxin by macrophages. Unlike nitric oxide (NO), nitrite cannot diffuse across bacterial membrane cells. The NirC protein acts as a specific channel to facilitate the transport of nitrite into Salmonella and E. coli cells for nitrogen metabolism and cytoplasmic detoxification. NirC is also required for the pathogenicity of Salmonella by downregulating the production of NO by the host macrophages. Based on an in vitro microarray that revealed the overexpression of the nirC gene in APEC strain SCI-07, we constructed a nirC-deficient SCI-07 strain (δ. nirC) and evaluated its virulence potential using in vivo and in vitro assays. The final cumulative mortalities caused by mutant and wild-type (WT) were similar; while the δ. nirC caused a gradual increase in the mortality rate during the seven days recorded, the WT caused mortality up to 24. h post-infection (hpi). Counts of the δ. nirC cells in the spleen, lung and liver were higher than those of the WT after 48. hpi but similar at 24. hpi. Although similar number of δ. nirC and WT cells was observed in macrophages at 3. hpi, there was higher number of δ. nirC cells at 16. hpi. The cell adhesion ability of the δ. nirC strain was about half the WT level in the presence and absence of alpha-. d-mannopyranoside. These results indicate that the nirC gene influences the pathogenicity of SCI-07 strain.

KW - APEC

KW - Escherichia coli

KW - NirC

KW - Pathogenicity

KW - Virulence

UR - http://www.scopus.com/inward/record.url?scp=84916607049&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84916607049&partnerID=8YFLogxK

U2 - 10.1016/j.vetmic.2014.11.015

DO - 10.1016/j.vetmic.2014.11.015

M3 - Article

C2 - 25487442

AN - SCOPUS:84916607049

VL - 175

SP - 123

EP - 131

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 1

ER -