Influenza A virus preferentially snatches noncoding RNA caps

Weifeng Gu, Glen R. Gallagher, Weiwei Dai, Ping Liu, Ruidong Li, Melanie I. Trombly, Don B. Gammon, Craig C. Mello, Jennifer P. Wang, Robert W. Finberg

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Influenza A virus (IAV) lacks the enzyme for adding 5' caps to its RNAs and snatches the 5' ends of host capped RNAs to prime transcription. Neither the preference of the host RNA sequences snatched nor the effect of cap-snatching on host processes is completely defined. Previous studies of influenza cap-snatching used poly(A)-selected RNAs from infected cells or relied on annotated host genes to define the snatched host RNAs, and thus lack details on many noncoding host RNAs including snRNAs, snoRNAs, and promoter-Associated capped small (cs)RNAs, which are made by "paused" Pol II during transcription initiation. In this study, we used a nonbiased technique, CapSeq, to identify host and viral-capped RNAs including nonpolyadenylated RNAs in the same samples, and investigated the substrate-product correlation between the host RNAs and the viral RNAs. We demonstrated that noncoding host RNAs, particularly U1 and U2, are the preferred cap-snatching source over mRNAs or pre-mRNAs. We also found that csRNAs are highly snatched by IAV. Because the functions of csRNAs remain mostly unknown, especially in somatic cells, our finding reveals that csRNAs at least play roles in the process of IAV infection. Our findings support a model where nascent RNAs including csRNAs are the preferred targets for cap-snatching by IAV and raise questions about how IAV might use snatching preferences to modulate host-mRNA splicing and transcription.

Original languageEnglish (US)
Pages (from-to)2067-2075
Number of pages9
JournalRNA
Volume21
Issue number12
DOIs
StatePublished - Jan 1 2015

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RNA Caps
Untranslated RNA
Influenza A virus
RNA
Viral RNA
Messenger RNA
Small Nucleolar RNA
RNA Precursors
Virus Diseases
Human Influenza

Keywords

  • Cap-snatching
  • Influenza
  • Noncoding RNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Gu, W., Gallagher, G. R., Dai, W., Liu, P., Li, R., Trombly, M. I., ... Finberg, R. W. (2015). Influenza A virus preferentially snatches noncoding RNA caps. RNA, 21(12), 2067-2075. https://doi.org/10.1261/rna.054221.115

Influenza A virus preferentially snatches noncoding RNA caps. / Gu, Weifeng; Gallagher, Glen R.; Dai, Weiwei; Liu, Ping; Li, Ruidong; Trombly, Melanie I.; Gammon, Don B.; Mello, Craig C.; Wang, Jennifer P.; Finberg, Robert W.

In: RNA, Vol. 21, No. 12, 01.01.2015, p. 2067-2075.

Research output: Contribution to journalArticle

Gu, W, Gallagher, GR, Dai, W, Liu, P, Li, R, Trombly, MI, Gammon, DB, Mello, CC, Wang, JP & Finberg, RW 2015, 'Influenza A virus preferentially snatches noncoding RNA caps', RNA, vol. 21, no. 12, pp. 2067-2075. https://doi.org/10.1261/rna.054221.115
Gu W, Gallagher GR, Dai W, Liu P, Li R, Trombly MI et al. Influenza A virus preferentially snatches noncoding RNA caps. RNA. 2015 Jan 1;21(12):2067-2075. https://doi.org/10.1261/rna.054221.115
Gu, Weifeng ; Gallagher, Glen R. ; Dai, Weiwei ; Liu, Ping ; Li, Ruidong ; Trombly, Melanie I. ; Gammon, Don B. ; Mello, Craig C. ; Wang, Jennifer P. ; Finberg, Robert W. / Influenza A virus preferentially snatches noncoding RNA caps. In: RNA. 2015 ; Vol. 21, No. 12. pp. 2067-2075.
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