Influenza virus hemagglutinin expression is polarized in cells infected with recombinant SV40 viruses carrying cloned hemagglutinin DNA

Michael G. Roth, Richard W. Compans, Lorraine Giusti, Alan R. Davis, Debi P. Nayak, Mary Jane Gething, Joe Sambrook

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Primary cell cultures of African Green monkey kidney (AGMK) contain polarized epithelial cells in which influenza virus matures predominantly at the apical surfaces above tight junctions. Influenza virus glycoproteins were found to be localized at the same membrane domain from which the virus budded. When polarized primary AGMK cells were infected with recombinant SV40 viruses containing DNA coding for either an influenza virus H1 or H2 subtype hemagglutinin (HA), the HA proteins were preferentially expressed at the apical surface in a manner identical to that observed in influenza virus-infected cells. Thus, cellular mechanisms for sorting membrane glycoproteins recognize some structural feature of the HA glycoprotein itself, and other viral proteins are not necessary for this process.

Original languageEnglish (US)
Pages (from-to)435-443
Number of pages9
JournalCell
Volume33
Issue number2
DOIs
StatePublished - 1983

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Simian virus 40
Hemagglutinins
Orthomyxoviridae
Viruses
Cercopithecus aethiops
DNA
Glycoproteins
Kidney
Primary Cell Culture
Tight Junctions
Membrane Glycoproteins
Viral Proteins
Epithelial Cells
Sorting
Cell culture
Membranes
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Influenza virus hemagglutinin expression is polarized in cells infected with recombinant SV40 viruses carrying cloned hemagglutinin DNA. / Roth, Michael G.; Compans, Richard W.; Giusti, Lorraine; Davis, Alan R.; Nayak, Debi P.; Gething, Mary Jane; Sambrook, Joe.

In: Cell, Vol. 33, No. 2, 1983, p. 435-443.

Research output: Contribution to journalArticle

Roth, Michael G. ; Compans, Richard W. ; Giusti, Lorraine ; Davis, Alan R. ; Nayak, Debi P. ; Gething, Mary Jane ; Sambrook, Joe. / Influenza virus hemagglutinin expression is polarized in cells infected with recombinant SV40 viruses carrying cloned hemagglutinin DNA. In: Cell. 1983 ; Vol. 33, No. 2. pp. 435-443.
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