Inherited coding variants at the CDKN2A locus influence susceptibility to acute lymphoblastic leukaemia in children

Heng Xu, Hui Zhang, Wenjian Yang, Rachita Yadav, Alanna C. Morrison, Maoxiang Qian, Meenakshi Devidas, Yu Liu, Virginia Perez-Andreu, Xujie Zhao, Julie M. Gastier-Foster, Philip J. Lupo, Geoff Neale, Elizabeth Raetz, Eric Larsen, W. Paul Bowman, William L. Carroll, Naomi Winick, Richard Williams, Torben HansenJens Christian Holm, Elaine Mardis, Robert Fulton, Ching Hon Pui, Jinghui Zhang, Charles G. Mullighan, William E. Evans, Stephen P. Hunger, Ramneek Gupta, Kjeld Schmiegelow, Mignon L. Loh, Mary V. Relling, Jun J. Yang

Research output: Contribution to journalArticle

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Abstract

There is increasing evidence from genome-wide association studies for a strong inherited genetic basis of susceptibility to acute lymphoblastic leukaemia (ALL) in children, yet the effects of protein-coding variants on ALL risk have not been systematically evaluated. Here we show a missense variant in CDKN2A associated with the development of ALL at genome-wide significance (rs3731249, P=9.4 × 10-23, odds ratio=2.23). Functional studies indicate that this hypomorphic variant results in reduced tumour suppressor function of p16 INK4A, increases the susceptibility to leukaemic transformation of haematopoietic progenitor cells, and is preferentially retained in ALL tumour cells. Resequencing the CDKN2A-CDKN2B locus in 2,407 childhood ALL cases reveals 19 additional putative functional germline variants. These results provide direct functional evidence for the influence of inherited genetic variation on ALL risk, highlighting the important and complex roles of CDKN2A-CDKN2B tumour suppressors in leukaemogenesis.

Original languageEnglish (US)
Article number7553
JournalNature Communications
Volume6
DOIs
StatePublished - Jun 24 2015

Fingerprint

leukemias
loci
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Tumors
coding
magnetic permeability
Genes
Cyclin-Dependent Kinase Inhibitor p16
suppressors
tumors
genome
Cells
Neoplasms
Genome-Wide Association Study
Genetic Predisposition to Disease
Hematopoietic Stem Cells
Proteins
Odds Ratio
Genome
proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Inherited coding variants at the CDKN2A locus influence susceptibility to acute lymphoblastic leukaemia in children. / Xu, Heng; Zhang, Hui; Yang, Wenjian; Yadav, Rachita; Morrison, Alanna C.; Qian, Maoxiang; Devidas, Meenakshi; Liu, Yu; Perez-Andreu, Virginia; Zhao, Xujie; Gastier-Foster, Julie M.; Lupo, Philip J.; Neale, Geoff; Raetz, Elizabeth; Larsen, Eric; Bowman, W. Paul; Carroll, William L.; Winick, Naomi; Williams, Richard; Hansen, Torben; Holm, Jens Christian; Mardis, Elaine; Fulton, Robert; Pui, Ching Hon; Zhang, Jinghui; Mullighan, Charles G.; Evans, William E.; Hunger, Stephen P.; Gupta, Ramneek; Schmiegelow, Kjeld; Loh, Mignon L.; Relling, Mary V.; Yang, Jun J.

In: Nature Communications, Vol. 6, 7553, 24.06.2015.

Research output: Contribution to journalArticle

Xu, H, Zhang, H, Yang, W, Yadav, R, Morrison, AC, Qian, M, Devidas, M, Liu, Y, Perez-Andreu, V, Zhao, X, Gastier-Foster, JM, Lupo, PJ, Neale, G, Raetz, E, Larsen, E, Bowman, WP, Carroll, WL, Winick, N, Williams, R, Hansen, T, Holm, JC, Mardis, E, Fulton, R, Pui, CH, Zhang, J, Mullighan, CG, Evans, WE, Hunger, SP, Gupta, R, Schmiegelow, K, Loh, ML, Relling, MV & Yang, JJ 2015, 'Inherited coding variants at the CDKN2A locus influence susceptibility to acute lymphoblastic leukaemia in children', Nature Communications, vol. 6, 7553. https://doi.org/10.1038/ncomms8553
Xu, Heng ; Zhang, Hui ; Yang, Wenjian ; Yadav, Rachita ; Morrison, Alanna C. ; Qian, Maoxiang ; Devidas, Meenakshi ; Liu, Yu ; Perez-Andreu, Virginia ; Zhao, Xujie ; Gastier-Foster, Julie M. ; Lupo, Philip J. ; Neale, Geoff ; Raetz, Elizabeth ; Larsen, Eric ; Bowman, W. Paul ; Carroll, William L. ; Winick, Naomi ; Williams, Richard ; Hansen, Torben ; Holm, Jens Christian ; Mardis, Elaine ; Fulton, Robert ; Pui, Ching Hon ; Zhang, Jinghui ; Mullighan, Charles G. ; Evans, William E. ; Hunger, Stephen P. ; Gupta, Ramneek ; Schmiegelow, Kjeld ; Loh, Mignon L. ; Relling, Mary V. ; Yang, Jun J. / Inherited coding variants at the CDKN2A locus influence susceptibility to acute lymphoblastic leukaemia in children. In: Nature Communications. 2015 ; Vol. 6.
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