Inhibition by cellular vacuolar atpase impairs human papillomavirus uncoating and infection

Konstantin H. MÜller, Gilles A. Spoden, Konstanze D. Scheffer, Regina Brunnhöfer, Jef K. De Brabander, Martin E. Maier, Luise Florin, Claude P. Muller

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Several viruses, including human papillomaviruses, depend on endosomal acidification for successful infection. Hence, the multisubunit enzyme vacuolar ATPase (V-ATPase), which is mainly responsible for endosome acidification in the cell, represents an attractive target for antiviral strategies. In the present study, we show that V-ATPase is required for human papillomavirus (HPV) infection and that uncoating/disassembly but not endocytosis is affected by V-ATPase inhibition. The infection inhibitory potencies of saliphenylhalamide, a proven V-ATPase inhibitor, and its derivatives, as well as those of other V-ATPase inhibitors, were analyzed on different HPV types in relevant cell lines. Variation in the selectivity indices among V-ATPase inhibitors was high, while variation for the same inhibitor against different HPV subtypes was low, indicating that broad-spectrum anti-HPV activity can be provided.

Original languageEnglish (US)
Pages (from-to)2905-2911
Number of pages7
JournalAntimicrobial Agents and Chemotherapy
Volume58
Issue number5
DOIs
StatePublished - 2014

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Vacuolar Proton-Translocating ATPases
Papillomavirus Infections
Papillomaviridae
Endosomes
Endocytosis
Infection
Human Activities
Antiviral Agents
Cell Line
Enzymes

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

MÜller, K. H., Spoden, G. A., Scheffer, K. D., Brunnhöfer, R., De Brabander, J. K., Maier, M. E., ... Muller, C. P. (2014). Inhibition by cellular vacuolar atpase impairs human papillomavirus uncoating and infection. Antimicrobial Agents and Chemotherapy, 58(5), 2905-2911. https://doi.org/10.1128/AAC.02284-13

Inhibition by cellular vacuolar atpase impairs human papillomavirus uncoating and infection. / MÜller, Konstantin H.; Spoden, Gilles A.; Scheffer, Konstanze D.; Brunnhöfer, Regina; De Brabander, Jef K.; Maier, Martin E.; Florin, Luise; Muller, Claude P.

In: Antimicrobial Agents and Chemotherapy, Vol. 58, No. 5, 2014, p. 2905-2911.

Research output: Contribution to journalArticle

MÜller, KH, Spoden, GA, Scheffer, KD, Brunnhöfer, R, De Brabander, JK, Maier, ME, Florin, L & Muller, CP 2014, 'Inhibition by cellular vacuolar atpase impairs human papillomavirus uncoating and infection', Antimicrobial Agents and Chemotherapy, vol. 58, no. 5, pp. 2905-2911. https://doi.org/10.1128/AAC.02284-13
MÜller, Konstantin H. ; Spoden, Gilles A. ; Scheffer, Konstanze D. ; Brunnhöfer, Regina ; De Brabander, Jef K. ; Maier, Martin E. ; Florin, Luise ; Muller, Claude P. / Inhibition by cellular vacuolar atpase impairs human papillomavirus uncoating and infection. In: Antimicrobial Agents and Chemotherapy. 2014 ; Vol. 58, No. 5. pp. 2905-2911.
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