Inhibition by SKF38393 of GABA-activated currents in rat DRG neurons

Qin Li, Qin Wen Wang, Zhi Wang Li

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Experiments were performed on neurons freshly isolated from rat DRG using whole-cell patch-clamp techniques. The majority of the neurons examined were sensitive to GABA (60/70) in the concentration range from 10-6 to 10 -3 mol/L, showing obvious desensitization. In the 60 GABA - sensitive cells, responses induced by selective agonist of dopamine D1 receptor SKF38393 [ ( ± ) SKF38393HCL] manifested three types: (1) outward current (7/60); (2) inward current (5/60) and (3) no detectable response (48/60). As compared with GABA-activated current, the amplitude of SKF38393-activated current are smaller and showed no apparent desensitization. When the neurons were treated with SKF38393 prior to application of GABA for 30 s, the GABA-activated current in majority of the cells (53/60) was inhibited, while the remaining six showed no effect. The effect of SKF38393 was dose dependent. That is, with SKF38393 at concentration of 10-7, 10-6, 10-5 and 10-4 mol/L, the GABA-activated current was inhibited by (24.8 ± 2.6)% (n = 7), (26.8 ± 1.5)% (n = 7), (35.6 ± 1.2)% (n =8) and (45.6 ± 2.3)% (n = 8) respectively. By intracellular application of 10-4 mol/L H-7, a potent inhibitor of protein kinase, the inhibitory effect of SKF38393 was abolished completely, a results suggesting that the inhibition by SKF38393 of the GABA-activated current might be a result of intracellular phosphorylation of GABAA receptor.

Original languageEnglish (US)
Pages (from-to)280-288
Number of pages9
JournalActa Physiologica Sinica
Volume50
Issue number3
StatePublished - 1998

Fingerprint

2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
Diagnosis-Related Groups
gamma-Aminobutyric Acid
Neurons
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Dopamine D1 Receptors
Patch-Clamp Techniques
GABA-A Receptors
Protein Kinase Inhibitors
Phosphorylation

Keywords

  • Dopamine D receptor agonist SKF3839
  • Dorsal root ganalion
  • GABA
  • Inhibition
  • Whole-cell patch-clamp recording

ASJC Scopus subject areas

  • Physiology

Cite this

Inhibition by SKF38393 of GABA-activated currents in rat DRG neurons. / Li, Qin; Wang, Qin Wen; Li, Zhi Wang.

In: Acta Physiologica Sinica, Vol. 50, No. 3, 1998, p. 280-288.

Research output: Contribution to journalArticle

Li, Q, Wang, QW & Li, ZW 1998, 'Inhibition by SKF38393 of GABA-activated currents in rat DRG neurons', Acta Physiologica Sinica, vol. 50, no. 3, pp. 280-288.
Li, Qin ; Wang, Qin Wen ; Li, Zhi Wang. / Inhibition by SKF38393 of GABA-activated currents in rat DRG neurons. In: Acta Physiologica Sinica. 1998 ; Vol. 50, No. 3. pp. 280-288.
@article{2a3be2371fa947b29fd338b573547e80,
title = "Inhibition by SKF38393 of GABA-activated currents in rat DRG neurons",
abstract = "Experiments were performed on neurons freshly isolated from rat DRG using whole-cell patch-clamp techniques. The majority of the neurons examined were sensitive to GABA (60/70) in the concentration range from 10-6 to 10 -3 mol/L, showing obvious desensitization. In the 60 GABA - sensitive cells, responses induced by selective agonist of dopamine D1 receptor SKF38393 [ ( ± ) SKF38393HCL] manifested three types: (1) outward current (7/60); (2) inward current (5/60) and (3) no detectable response (48/60). As compared with GABA-activated current, the amplitude of SKF38393-activated current are smaller and showed no apparent desensitization. When the neurons were treated with SKF38393 prior to application of GABA for 30 s, the GABA-activated current in majority of the cells (53/60) was inhibited, while the remaining six showed no effect. The effect of SKF38393 was dose dependent. That is, with SKF38393 at concentration of 10-7, 10-6, 10-5 and 10-4 mol/L, the GABA-activated current was inhibited by (24.8 ± 2.6){\%} (n = 7), (26.8 ± 1.5){\%} (n = 7), (35.6 ± 1.2){\%} (n =8) and (45.6 ± 2.3){\%} (n = 8) respectively. By intracellular application of 10-4 mol/L H-7, a potent inhibitor of protein kinase, the inhibitory effect of SKF38393 was abolished completely, a results suggesting that the inhibition by SKF38393 of the GABA-activated current might be a result of intracellular phosphorylation of GABAA receptor.",
keywords = "Dopamine D receptor agonist SKF3839, Dorsal root ganalion, GABA, Inhibition, Whole-cell patch-clamp recording",
author = "Qin Li and Wang, {Qin Wen} and Li, {Zhi Wang}",
year = "1998",
language = "English (US)",
volume = "50",
pages = "280--288",
journal = "Sheng li xue bao : [Acta physiologica Sinica]",
issn = "0371-0874",
publisher = "Kexue Chubaneshe/Science Press",
number = "3",

}

TY - JOUR

T1 - Inhibition by SKF38393 of GABA-activated currents in rat DRG neurons

AU - Li, Qin

AU - Wang, Qin Wen

AU - Li, Zhi Wang

PY - 1998

Y1 - 1998

N2 - Experiments were performed on neurons freshly isolated from rat DRG using whole-cell patch-clamp techniques. The majority of the neurons examined were sensitive to GABA (60/70) in the concentration range from 10-6 to 10 -3 mol/L, showing obvious desensitization. In the 60 GABA - sensitive cells, responses induced by selective agonist of dopamine D1 receptor SKF38393 [ ( ± ) SKF38393HCL] manifested three types: (1) outward current (7/60); (2) inward current (5/60) and (3) no detectable response (48/60). As compared with GABA-activated current, the amplitude of SKF38393-activated current are smaller and showed no apparent desensitization. When the neurons were treated with SKF38393 prior to application of GABA for 30 s, the GABA-activated current in majority of the cells (53/60) was inhibited, while the remaining six showed no effect. The effect of SKF38393 was dose dependent. That is, with SKF38393 at concentration of 10-7, 10-6, 10-5 and 10-4 mol/L, the GABA-activated current was inhibited by (24.8 ± 2.6)% (n = 7), (26.8 ± 1.5)% (n = 7), (35.6 ± 1.2)% (n =8) and (45.6 ± 2.3)% (n = 8) respectively. By intracellular application of 10-4 mol/L H-7, a potent inhibitor of protein kinase, the inhibitory effect of SKF38393 was abolished completely, a results suggesting that the inhibition by SKF38393 of the GABA-activated current might be a result of intracellular phosphorylation of GABAA receptor.

AB - Experiments were performed on neurons freshly isolated from rat DRG using whole-cell patch-clamp techniques. The majority of the neurons examined were sensitive to GABA (60/70) in the concentration range from 10-6 to 10 -3 mol/L, showing obvious desensitization. In the 60 GABA - sensitive cells, responses induced by selective agonist of dopamine D1 receptor SKF38393 [ ( ± ) SKF38393HCL] manifested three types: (1) outward current (7/60); (2) inward current (5/60) and (3) no detectable response (48/60). As compared with GABA-activated current, the amplitude of SKF38393-activated current are smaller and showed no apparent desensitization. When the neurons were treated with SKF38393 prior to application of GABA for 30 s, the GABA-activated current in majority of the cells (53/60) was inhibited, while the remaining six showed no effect. The effect of SKF38393 was dose dependent. That is, with SKF38393 at concentration of 10-7, 10-6, 10-5 and 10-4 mol/L, the GABA-activated current was inhibited by (24.8 ± 2.6)% (n = 7), (26.8 ± 1.5)% (n = 7), (35.6 ± 1.2)% (n =8) and (45.6 ± 2.3)% (n = 8) respectively. By intracellular application of 10-4 mol/L H-7, a potent inhibitor of protein kinase, the inhibitory effect of SKF38393 was abolished completely, a results suggesting that the inhibition by SKF38393 of the GABA-activated current might be a result of intracellular phosphorylation of GABAA receptor.

KW - Dopamine D receptor agonist SKF3839

KW - Dorsal root ganalion

KW - GABA

KW - Inhibition

KW - Whole-cell patch-clamp recording

UR - http://www.scopus.com/inward/record.url?scp=0347571048&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0347571048&partnerID=8YFLogxK

M3 - Article

C2 - 11324568

AN - SCOPUS:0347571048

VL - 50

SP - 280

EP - 288

JO - Sheng li xue bao : [Acta physiologica Sinica]

JF - Sheng li xue bao : [Acta physiologica Sinica]

SN - 0371-0874

IS - 3

ER -