Abstract
Experiments were performed on neurons freshly isolated from rat DRG using whole-cell patch-clamp techniques. The majority of the neurons examined were sensitive to GABA (60/70) in the concentration range from 10-6 to 10 -3 mol/L, showing obvious desensitization. In the 60 GABA - sensitive cells, responses induced by selective agonist of dopamine D1 receptor SKF38393 [ ( ± ) SKF38393HCL] manifested three types: (1) outward current (7/60); (2) inward current (5/60) and (3) no detectable response (48/60). As compared with GABA-activated current, the amplitude of SKF38393-activated current are smaller and showed no apparent desensitization. When the neurons were treated with SKF38393 prior to application of GABA for 30 s, the GABA-activated current in majority of the cells (53/60) was inhibited, while the remaining six showed no effect. The effect of SKF38393 was dose dependent. That is, with SKF38393 at concentration of 10-7, 10-6, 10-5 and 10-4 mol/L, the GABA-activated current was inhibited by (24.8 ± 2.6)% (n = 7), (26.8 ± 1.5)% (n = 7), (35.6 ± 1.2)% (n =8) and (45.6 ± 2.3)% (n = 8) respectively. By intracellular application of 10-4 mol/L H-7, a potent inhibitor of protein kinase, the inhibitory effect of SKF38393 was abolished completely, a results suggesting that the inhibition by SKF38393 of the GABA-activated current might be a result of intracellular phosphorylation of GABAA receptor.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 280-288 |
| Number of pages | 9 |
| Journal | Acta Physiologica Sinica |
| Volume | 50 |
| Issue number | 3 |
| State | Published - 1998 |
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Keywords
- Dopamine D receptor agonist SKF3839
- Dorsal root ganalion
- GABA
- Inhibition
- Whole-cell patch-clamp recording
ASJC Scopus subject areas
- Physiology
Cite this
Inhibition by SKF38393 of GABA-activated currents in rat DRG neurons. / Li, Qin; Wang, Qin Wen; Li, Zhi Wang.
In: Acta Physiologica Sinica, Vol. 50, No. 3, 1998, p. 280-288.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition by SKF38393 of GABA-activated currents in rat DRG neurons
AU - Li, Qin
AU - Wang, Qin Wen
AU - Li, Zhi Wang
PY - 1998
Y1 - 1998
N2 - Experiments were performed on neurons freshly isolated from rat DRG using whole-cell patch-clamp techniques. The majority of the neurons examined were sensitive to GABA (60/70) in the concentration range from 10-6 to 10 -3 mol/L, showing obvious desensitization. In the 60 GABA - sensitive cells, responses induced by selective agonist of dopamine D1 receptor SKF38393 [ ( ± ) SKF38393HCL] manifested three types: (1) outward current (7/60); (2) inward current (5/60) and (3) no detectable response (48/60). As compared with GABA-activated current, the amplitude of SKF38393-activated current are smaller and showed no apparent desensitization. When the neurons were treated with SKF38393 prior to application of GABA for 30 s, the GABA-activated current in majority of the cells (53/60) was inhibited, while the remaining six showed no effect. The effect of SKF38393 was dose dependent. That is, with SKF38393 at concentration of 10-7, 10-6, 10-5 and 10-4 mol/L, the GABA-activated current was inhibited by (24.8 ± 2.6)% (n = 7), (26.8 ± 1.5)% (n = 7), (35.6 ± 1.2)% (n =8) and (45.6 ± 2.3)% (n = 8) respectively. By intracellular application of 10-4 mol/L H-7, a potent inhibitor of protein kinase, the inhibitory effect of SKF38393 was abolished completely, a results suggesting that the inhibition by SKF38393 of the GABA-activated current might be a result of intracellular phosphorylation of GABAA receptor.
AB - Experiments were performed on neurons freshly isolated from rat DRG using whole-cell patch-clamp techniques. The majority of the neurons examined were sensitive to GABA (60/70) in the concentration range from 10-6 to 10 -3 mol/L, showing obvious desensitization. In the 60 GABA - sensitive cells, responses induced by selective agonist of dopamine D1 receptor SKF38393 [ ( ± ) SKF38393HCL] manifested three types: (1) outward current (7/60); (2) inward current (5/60) and (3) no detectable response (48/60). As compared with GABA-activated current, the amplitude of SKF38393-activated current are smaller and showed no apparent desensitization. When the neurons were treated with SKF38393 prior to application of GABA for 30 s, the GABA-activated current in majority of the cells (53/60) was inhibited, while the remaining six showed no effect. The effect of SKF38393 was dose dependent. That is, with SKF38393 at concentration of 10-7, 10-6, 10-5 and 10-4 mol/L, the GABA-activated current was inhibited by (24.8 ± 2.6)% (n = 7), (26.8 ± 1.5)% (n = 7), (35.6 ± 1.2)% (n =8) and (45.6 ± 2.3)% (n = 8) respectively. By intracellular application of 10-4 mol/L H-7, a potent inhibitor of protein kinase, the inhibitory effect of SKF38393 was abolished completely, a results suggesting that the inhibition by SKF38393 of the GABA-activated current might be a result of intracellular phosphorylation of GABAA receptor.
KW - Dopamine D receptor agonist SKF3839
KW - Dorsal root ganalion
KW - GABA
KW - Inhibition
KW - Whole-cell patch-clamp recording
UR - http://www.scopus.com/inward/record.url?scp=0347571048&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0347571048&partnerID=8YFLogxK
M3 - Article
C2 - 11324568
AN - SCOPUS:0347571048
VL - 50
SP - 280
EP - 288
JO - Sheng li xue bao : [Acta physiologica Sinica]
JF - Sheng li xue bao : [Acta physiologica Sinica]
SN - 0371-0874
IS - 3
ER -