Inhibition of adenylyl cyclase by G

Ronald Taussig, Jorge A. Iñiguez-Lluhi, Alfred G. Gilman

Research output: Contribution to journalArticle

297 Citations (Scopus)

Abstract

Evidence suggests that both α and βγ subunits of heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) inhibit adenylyl cyclase. Although type I adenylyl cyclase is inhibited directly by exogenous βγ, inhibition of adenylyl cyclase by G has not been convincingly demonstrated in vitro. Concentration-dependent inhibition of adenylyl cyclases by purified Giα subunits is described. Activated G but not G was effective, and myristoylation of G was required. The characteristics of the inhibitory effect were dependent on the type of adenylyl cyclase and the nature of the activator of the enzyme. The concentrations of G required to inhibit adenylyl cyclase were substantially higher than those normally thought to be relevant physiologically. However, analysis indicates that these concentrations may be relevant and reasonable.

Original languageEnglish (US)
Pages (from-to)218-221
Number of pages4
JournalScience
Volume261
Issue number5118
StatePublished - Jul 9 1993

Fingerprint

Adenylyl Cyclases
Enzyme Activators
GTP-Binding Proteins
Carrier Proteins

ASJC Scopus subject areas

  • General

Cite this

Taussig, R., Iñiguez-Lluhi, J. A., & Gilman, A. G. (1993). Inhibition of adenylyl cyclase by G Science, 261(5118), 218-221.

Inhibition of adenylyl cyclase by G . / Taussig, Ronald; Iñiguez-Lluhi, Jorge A.; Gilman, Alfred G.

In: Science, Vol. 261, No. 5118, 09.07.1993, p. 218-221.

Research output: Contribution to journalArticle

Taussig, R, Iñiguez-Lluhi, JA & Gilman, AG 1993, 'Inhibition of adenylyl cyclase by G ', Science, vol. 261, no. 5118, pp. 218-221.
Taussig R, Iñiguez-Lluhi JA, Gilman AG. Inhibition of adenylyl cyclase by G Science. 1993 Jul 9;261(5118):218-221.
Taussig, Ronald ; Iñiguez-Lluhi, Jorge A. ; Gilman, Alfred G. / Inhibition of adenylyl cyclase by G In: Science. 1993 ; Vol. 261, No. 5118. pp. 218-221.
@article{1202ed43847b4edfa441024b69bf1add,
title = "Inhibition of adenylyl cyclase by Giα",
abstract = "Evidence suggests that both α and βγ subunits of heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) inhibit adenylyl cyclase. Although type I adenylyl cyclase is inhibited directly by exogenous βγ, inhibition of adenylyl cyclase by Giα has not been convincingly demonstrated in vitro. Concentration-dependent inhibition of adenylyl cyclases by purified Giα subunits is described. Activated Giα but not Goα was effective, and myristoylation of Giα was required. The characteristics of the inhibitory effect were dependent on the type of adenylyl cyclase and the nature of the activator of the enzyme. The concentrations of Giα required to inhibit adenylyl cyclase were substantially higher than those normally thought to be relevant physiologically. However, analysis indicates that these concentrations may be relevant and reasonable.",
author = "Ronald Taussig and I{\~n}iguez-Lluhi, {Jorge A.} and Gilman, {Alfred G.}",
year = "1993",
month = "7",
day = "9",
language = "English (US)",
volume = "261",
pages = "218--221",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5118",

}

TY - JOUR

T1 - Inhibition of adenylyl cyclase by Giα

AU - Taussig, Ronald

AU - Iñiguez-Lluhi, Jorge A.

AU - Gilman, Alfred G.

PY - 1993/7/9

Y1 - 1993/7/9

N2 - Evidence suggests that both α and βγ subunits of heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) inhibit adenylyl cyclase. Although type I adenylyl cyclase is inhibited directly by exogenous βγ, inhibition of adenylyl cyclase by Giα has not been convincingly demonstrated in vitro. Concentration-dependent inhibition of adenylyl cyclases by purified Giα subunits is described. Activated Giα but not Goα was effective, and myristoylation of Giα was required. The characteristics of the inhibitory effect were dependent on the type of adenylyl cyclase and the nature of the activator of the enzyme. The concentrations of Giα required to inhibit adenylyl cyclase were substantially higher than those normally thought to be relevant physiologically. However, analysis indicates that these concentrations may be relevant and reasonable.

AB - Evidence suggests that both α and βγ subunits of heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) inhibit adenylyl cyclase. Although type I adenylyl cyclase is inhibited directly by exogenous βγ, inhibition of adenylyl cyclase by Giα has not been convincingly demonstrated in vitro. Concentration-dependent inhibition of adenylyl cyclases by purified Giα subunits is described. Activated Giα but not Goα was effective, and myristoylation of Giα was required. The characteristics of the inhibitory effect were dependent on the type of adenylyl cyclase and the nature of the activator of the enzyme. The concentrations of Giα required to inhibit adenylyl cyclase were substantially higher than those normally thought to be relevant physiologically. However, analysis indicates that these concentrations may be relevant and reasonable.

UR - http://www.scopus.com/inward/record.url?scp=0027291886&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027291886&partnerID=8YFLogxK

M3 - Article

VL - 261

SP - 218

EP - 221

JO - Science

JF - Science

SN - 0036-8075

IS - 5118

ER -