Inhibition of ATP-activated currents by met-Enk in isolated DRG neurons of the rat

Bao Lu Xia, Zi Zhen Wu, Xiao Li, Qin Li, Zhi Wang Li

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The present study aimed to explore modulation of ATP-activated currents (IATP) by met-Enk in rat DRG neurons. IATP were recorded using the whole-cell patch clamp technique. The majority of the neurons examined responded to ATP (90.0%, 45/50) with inward currents. In the 45 ATP sensitive neurons three kinds of responses to application of met-Enk were distinguished: (1) inward currents (29/45), (2) outward currents (9/45), and (3) no effect (7/45). Pretreatment with met-Enk (10-9-10-5 mol/L) suppressed IATP(10-4 mol/L) in 29 neurons responding to met-Enk with inward currents. The inhibition by met-Enk of IATP could be blocked by naloxone (10-7 mol/L) in a concentration-dependent manner. Met-Enk of 10-9, 10-8, 10-7, 10-6 and 10-5 mol/L suppressed IATP by 13.2 ± 5.4% (n = 5); 39.2 ± 8.6% (n = 8),54.1 ± 8.6% (n = 8),43.3 ± 7.9% (n = 7) and 43.1 ± 7.9% (n = 7) (mean ± MSE), respectively. A comparison of concentration - response relations of ATP with and without preapplication of met-Enk indicated that after pretreatment with met-Enk (10-7 mol/L) the curve shifted downward markedly with a decrease of 25% of the maxmum value of IATP and unchanged Kd value. The suppression of IATP by met-Enk was reversed as evidenced by intracellular dialysis of H-9 by using the repatch technique. Taken together, it is suggested that the inhibition by met-Enk of IATP is caused by activation of opiate receptor, which eventually results in phosphorylation of ATP receptor, mediated by modulation of G protein coupling and intracellular signal transduction.

Original languageEnglish (US)
Pages (from-to)205-208
Number of pages4
JournalActa Physiologica Sinica
Volume53
Issue number3
StatePublished - 2001

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Diagnosis-Related Groups
Adenosine Triphosphate
Neurons
Purinergic P2 Receptors
Opioid Receptors
Patch-Clamp Techniques
Naloxone
GTP-Binding Proteins
Dialysis
Signal Transduction
Phosphorylation

Keywords

  • ATP-activated current
  • DRG neuron
  • Inhibition
  • Met-enkephaline
  • Rat
  • Whole-cell patch clamp technique

ASJC Scopus subject areas

  • Physiology

Cite this

Xia, B. L., Wu, Z. Z., Li, X., Li, Q., & Li, Z. W. (2001). Inhibition of ATP-activated currents by met-Enk in isolated DRG neurons of the rat. Acta Physiologica Sinica, 53(3), 205-208.

Inhibition of ATP-activated currents by met-Enk in isolated DRG neurons of the rat. / Xia, Bao Lu; Wu, Zi Zhen; Li, Xiao; Li, Qin; Li, Zhi Wang.

In: Acta Physiologica Sinica, Vol. 53, No. 3, 2001, p. 205-208.

Research output: Contribution to journalArticle

Xia, BL, Wu, ZZ, Li, X, Li, Q & Li, ZW 2001, 'Inhibition of ATP-activated currents by met-Enk in isolated DRG neurons of the rat', Acta Physiologica Sinica, vol. 53, no. 3, pp. 205-208.
Xia, Bao Lu ; Wu, Zi Zhen ; Li, Xiao ; Li, Qin ; Li, Zhi Wang. / Inhibition of ATP-activated currents by met-Enk in isolated DRG neurons of the rat. In: Acta Physiologica Sinica. 2001 ; Vol. 53, No. 3. pp. 205-208.
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abstract = "The present study aimed to explore modulation of ATP-activated currents (IATP) by met-Enk in rat DRG neurons. IATP were recorded using the whole-cell patch clamp technique. The majority of the neurons examined responded to ATP (90.0{\%}, 45/50) with inward currents. In the 45 ATP sensitive neurons three kinds of responses to application of met-Enk were distinguished: (1) inward currents (29/45), (2) outward currents (9/45), and (3) no effect (7/45). Pretreatment with met-Enk (10-9-10-5 mol/L) suppressed IATP(10-4 mol/L) in 29 neurons responding to met-Enk with inward currents. The inhibition by met-Enk of IATP could be blocked by naloxone (10-7 mol/L) in a concentration-dependent manner. Met-Enk of 10-9, 10-8, 10-7, 10-6 and 10-5 mol/L suppressed IATP by 13.2 ± 5.4{\%} (n = 5); 39.2 ± 8.6{\%} (n = 8),54.1 ± 8.6{\%} (n = 8),43.3 ± 7.9{\%} (n = 7) and 43.1 ± 7.9{\%} (n = 7) (mean ± MSE), respectively. A comparison of concentration - response relations of ATP with and without preapplication of met-Enk indicated that after pretreatment with met-Enk (10-7 mol/L) the curve shifted downward markedly with a decrease of 25{\%} of the maxmum value of IATP and unchanged Kd value. The suppression of IATP by met-Enk was reversed as evidenced by intracellular dialysis of H-9 by using the repatch technique. Taken together, it is suggested that the inhibition by met-Enk of IATP is caused by activation of opiate receptor, which eventually results in phosphorylation of ATP receptor, mediated by modulation of G protein coupling and intracellular signal transduction.",
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