Inhibition of bacterial FMN transferase: A potential avenue for countering antimicrobial resistance

Ranjit K. Deka, Akanksha Deka, Wei Z. Liu, Michael V. Norgard, Chad A. Brautigam

Research output: Contribution to journalArticlepeer-review

Abstract

Antibiotic resistance is a challenge for the control of bacterial infections. In an effort to explore unconventional avenues for antibacterial drug development, we focused on the FMN-transferase activity of the enzyme Ftp from the syphilis spirochete, Treponema pallidum (Ftp_Tp). This enzyme, which is only found in prokaryotes and trypanosomatids, post-translationally modifies proteins in the periplasm, covalently linking FMN (from FAD) to proteins that typically are important for establishing an essential electrochemical gradient across the cytoplasmic membrane. As such, Ftp inhibitors potentially represent a new class of antimicrobials. Previously, we showed that AMP is both a product of the Ftp_tp-catalyzed reaction and an inhibitor of the enzyme. As a preliminary step in exploiting this property to develop a novel Ftp_Tp inhibitor, we have used structural and solution studies to examine the inhibitory and enzyme-binding properties of several adenine-based nucleosides, with particular focus on the 2-position of the purine ring. Implications for future drug design are discussed.

Original languageEnglish (US)
Pages (from-to)545-551
Number of pages7
JournalProtein Science
Volume31
Issue number2
DOIs
StatePublished - Feb 2022

Keywords

  • ADP
  • FMN transferase
  • X-ray crystallography
  • antibiotics
  • enzyme inhibitor
  • flavin adenine dinucleotide
  • microscale thermophoresis
  • post-translational modification

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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