Inhibition of bacterial growth by peptide-conjugated morpholino oligomers

Seth M. Daly, Carolyn R. Sturge, David E. Greenberg

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

Morpholino oligomers (MOs) are antisense molecules designed for sequence-specific binding of target mRNA. In bacteria, inhibition is hypothesized to occur by preventing translation initiation. Cell-penetrating peptides may be conjugated to the 5′- or 3′-termini of an MO to enhance cellular entry and therefore inhibition. Here we describe the three standard microbiological assays to assess in vitro antibacterial MO efficacy.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages115-122
Number of pages8
Volume1565
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1565
ISSN (Print)10643745

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Keywords

  • Antisense
  • Checkerboard synergy
  • Minimum bactericidal concentration (MBC)
  • Minimum inhibitory concentration (MIC)
  • Morpholino oligomers (MOs)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Daly, S. M., Sturge, C. R., & Greenberg, D. E. (2017). Inhibition of bacterial growth by peptide-conjugated morpholino oligomers. In Methods in Molecular Biology (Vol. 1565, pp. 115-122). (Methods in Molecular Biology; Vol. 1565). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6817-6_10