Inhibition of cardiac lipoprotein utilization by transgenic overexpression of Angptl4 in the heart

Xinxin Yu, Shawn C. Burgess, Hongfei Ge, Kenny K. Wong, R. Haris Nassem, Daniel J. Garry, A. Dean Sherry, Craig R. Malloy, Joel P. Berger, Cai Li

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

To investigate the role of Angptl4, an inhibitor of lipoprotein lipase that is induced by >3-fold in the heart after rosiglitazone treatment, we generated transgenic mice that overexpress Angptl4 in the heart (MHC-Angptl4). We show that MHC-Angptl4 mice exhibit cardiac-restricted expression of the transgene and inhibition of cardiac lipoprotein lipase (LPL) activity. However, LPL activities in other tissues or that released into plasma by heparin are not affected. In addition, MHC-Angptl4 mice also exhibit hypertriglyceridemia after 6 h of fasting. We use echocardiography to show that MHC-Angptl4 mice develop left-ventricular dysfunction. Comparison of the metabolic profiles of isolated working hearts demonstrates that cardiac impairment in MHC-Angptl4 mice is positively associated with decreased triglyceride (TG) utilization. When bred to transgenic mice that overexpress acyl-CoA synthetase in the heart, a strain that exhibits elevated cardiac TG accumulation, cardiac TG content in double transgenic mice is reversed to that of wild-type mice. Taken together, our data support the hypothesis that induction of Angptl4 in the heart inhibits lipoprotein-derived fatty acid delivery. This mouse model will be useful to elucidate the role of reduced fatty acid supply in the pathogenesis of heart failure and related disorders.

Original languageEnglish (US)
Pages (from-to)1767-1772
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number5
DOIs
StatePublished - Feb 1 2005

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Lipoproteins
Lipoprotein Lipase
Transgenic Mice
Triglycerides
rosiglitazone
Fatty Acids
Coenzyme A Ligases
Metabolome
Hypertriglyceridemia
Left Ventricular Dysfunction
Transgenes
Echocardiography
Heparin
Inhibition (Psychology)
Fasting
Heart Failure

Keywords

  • Cardiomyopathy
  • Lipase
  • Nuclear receptor

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Inhibition of cardiac lipoprotein utilization by transgenic overexpression of Angptl4 in the heart. / Yu, Xinxin; Burgess, Shawn C.; Ge, Hongfei; Wong, Kenny K.; Nassem, R. Haris; Garry, Daniel J.; Sherry, A. Dean; Malloy, Craig R.; Berger, Joel P.; Li, Cai.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 5, 01.02.2005, p. 1767-1772.

Research output: Contribution to journalArticle

Yu, Xinxin ; Burgess, Shawn C. ; Ge, Hongfei ; Wong, Kenny K. ; Nassem, R. Haris ; Garry, Daniel J. ; Sherry, A. Dean ; Malloy, Craig R. ; Berger, Joel P. ; Li, Cai. / Inhibition of cardiac lipoprotein utilization by transgenic overexpression of Angptl4 in the heart. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 5. pp. 1767-1772.
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