Inhibition of EZH2 expression is associated with the proliferation, apoptosis, and migration of SW620 colorectal cancer cells in vitro

Song Bing He, Hao Zhou, Jian Zhou, Guo Qiang Zhou, Tuo Han, Dai Wei Wan, Wen Gu, Lin Gao, Yi Zhang, Xiao Feng Xue, Li Feng Zhang, Min Fei, Shui Qing Hu, Xiao Dong Yang, Xin Guo Zhu, Liang Wang, De Chun Li

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Epigenetic changes have been recently recognized as important in human cancers. Enhancer of zeste homologue 2 gene (EZH2) has been shown the overexpression in various human cancers, consistent with a straightforward role of EZH2 as an oncogene, but its function in carcinogenesis is partly contradictory. The role of EZH2 in development of human colorectal cancer (CRC) has not yet been clarified. In the present study, we observed up-regulation of EZH2 expression in tumor tissues from CRC patients. The expression of EZH2 in CRC cell lines is consistent with the trend in cancer tissues using reverse transcription polymerase chain reaction (RT-PCR). We showed that TNM stage and lymph node metastasis in CRC patients are significantly correlated with EZH2 expression levels. EZH2 level of transcription and protein is inhibited by small interfering RNA (siRNA). More importantly, EZH2-siRNA inhibits the proliferation and migration of SW620 cells while promoting their apoptosis, and inducing G0/G1 cell cycle arrest of SW620 cells. Collectively, our results suggest that upregulated EZH2 expression may contribute to the progression of the patients with CRC. A comprehensive study of epigenetic mechanisms and the relevance of EZH2 in CRC is important for fully understanding this disease and as a basis for developing new treatment options in patients with CRC.

Original languageEnglish (US)
Pages (from-to)458-466
Number of pages9
JournalExperimental Biology and Medicine
Volume240
Issue number4
DOIs
StatePublished - Jan 1 2015

Fingerprint

Gene expression
Colorectal Neoplasms
Genes
Cells
Apoptosis
Gene Expression
Transcription
Small Interfering RNA
Tissue
Epigenomics
Neoplasms
Polymerase chain reaction
Inhibition (Psychology)
Enhancer of Zeste Homolog 2 Protein
G1 Phase Cell Cycle Checkpoints
Tumors
Human Development
Oncogenes
Reverse Transcription
Cell Movement

Keywords

  • apoptosis
  • colorectal cancer
  • EZH2
  • migration
  • proliferation
  • small interfering RNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Inhibition of EZH2 expression is associated with the proliferation, apoptosis, and migration of SW620 colorectal cancer cells in vitro. / He, Song Bing; Zhou, Hao; Zhou, Jian; Zhou, Guo Qiang; Han, Tuo; Wan, Dai Wei; Gu, Wen; Gao, Lin; Zhang, Yi; Xue, Xiao Feng; Zhang, Li Feng; Fei, Min; Hu, Shui Qing; Yang, Xiao Dong; Zhu, Xin Guo; Wang, Liang; Li, De Chun.

In: Experimental Biology and Medicine, Vol. 240, No. 4, 01.01.2015, p. 458-466.

Research output: Contribution to journalArticle

He, SB, Zhou, H, Zhou, J, Zhou, GQ, Han, T, Wan, DW, Gu, W, Gao, L, Zhang, Y, Xue, XF, Zhang, LF, Fei, M, Hu, SQ, Yang, XD, Zhu, XG, Wang, L & Li, DC 2015, 'Inhibition of EZH2 expression is associated with the proliferation, apoptosis, and migration of SW620 colorectal cancer cells in vitro', Experimental Biology and Medicine, vol. 240, no. 4, pp. 458-466. https://doi.org/10.1177/1535370214542215
He, Song Bing ; Zhou, Hao ; Zhou, Jian ; Zhou, Guo Qiang ; Han, Tuo ; Wan, Dai Wei ; Gu, Wen ; Gao, Lin ; Zhang, Yi ; Xue, Xiao Feng ; Zhang, Li Feng ; Fei, Min ; Hu, Shui Qing ; Yang, Xiao Dong ; Zhu, Xin Guo ; Wang, Liang ; Li, De Chun. / Inhibition of EZH2 expression is associated with the proliferation, apoptosis, and migration of SW620 colorectal cancer cells in vitro. In: Experimental Biology and Medicine. 2015 ; Vol. 240, No. 4. pp. 458-466.
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AU - Han, Tuo

AU - Wan, Dai Wei

AU - Gu, Wen

AU - Gao, Lin

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AU - Zhang, Li Feng

AU - Fei, Min

AU - Hu, Shui Qing

AU - Yang, Xiao Dong

AU - Zhu, Xin Guo

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