Inhibition of fatty acid oxidation activates transforming growth factor-beta in cerebrospinal fluid and decreases spontaneous motor activity

Teppei Fujikawa, Ryo Fujita, Yoko Iwaki, Shigenobu Matsumura, Tohru Fushiki, Kazuo Inoue

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have previously reported that transforming growth factor (TGF)-beta in the cerebrospinal fluid (CSF) is involved in the mechanism underlying the regulation of spontaneous motor activity (SMA) by the central nervous system after exercise. However, it remained unclear what physiological condition triggers the activation of TGF-beta. We hypothesized that the shortage of energy derived from fatty acid (FA) oxidation observed in the early phase of exercise activated TGF-beta in the CSF. To test this hypothesis, we investigated whether mercaptoacetate (MA), an inhibitor of FA oxidation, could induce an activation of TGF-beta in the CSF and a decrease in SMA. Intraperitoneal (i.p.) administration of MA activated TGF-beta in CSF in rats and depressed SMA; 2-deoxyglucose, an inhibitor of carbohydrate oxidation, on the other hand, depressed SMA but failed to activate CSF TGF-beta. Intracisternal administration of anti-TGF-beta antibody abolished the depressive effect of MA on SMA. We also found that the depression of SMA and the activation of TGF-beta in the CSF by i.p. MA administration were eliminated by vagotomy. Our data suggest that TGF-beta in the CSF is activated by the inhibition of FA oxidation via the vagus nerve and that this subsequently induces depression of SMA.

Original languageEnglish (US)
Pages (from-to)370-375
Number of pages6
JournalPhysiology and Behavior
Volume101
Issue number3
DOIs
StatePublished - Oct 2010

Fingerprint

Transforming Growth Factor beta
Cerebrospinal Fluid
Motor Activity
Fatty Acids
Inhibition (Psychology)
Vagus Nerve
Vagotomy
Deoxyglucose
Central Nervous System
Carbohydrates
Antibodies

Keywords

  • 2-deoxyglucose
  • Exercise
  • Fatty acid oxidation
  • Mercaptoacetate
  • TGF-beta
  • Vagotomy

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Experimental and Cognitive Psychology

Cite this

Inhibition of fatty acid oxidation activates transforming growth factor-beta in cerebrospinal fluid and decreases spontaneous motor activity. / Fujikawa, Teppei; Fujita, Ryo; Iwaki, Yoko; Matsumura, Shigenobu; Fushiki, Tohru; Inoue, Kazuo.

In: Physiology and Behavior, Vol. 101, No. 3, 10.2010, p. 370-375.

Research output: Contribution to journalArticle

Fujikawa, Teppei ; Fujita, Ryo ; Iwaki, Yoko ; Matsumura, Shigenobu ; Fushiki, Tohru ; Inoue, Kazuo. / Inhibition of fatty acid oxidation activates transforming growth factor-beta in cerebrospinal fluid and decreases spontaneous motor activity. In: Physiology and Behavior. 2010 ; Vol. 101, No. 3. pp. 370-375.
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