Inhibition of linoleic acid oxidation by 12-lipoxygenase from porcine leucocytes by linoleyl-hydroxamic acid

O. V. Kharchenko, V. N. Cernjuk, I. A. Butovich

Research output: Contribution to journalArticle

Abstract

Linoleic acid oxidation by 12-lipoxygenase from porcine leukocytes has been studied as affected by linoleyl-hydroxamic acid. Linoleyl-hydroxamic acid has been found to be an effective inhibitor of porcine leucocyte 12-lipoxygenase. Aerobic preincubation of 12-lipoxygenase with 0, 1-6 μM of linoleyl-hydroxamic acid led to a time- and dose-dependent inhibition of the enzyme. The inhibitor's concentration able to induce a 50% loss of the enzyme activity with and without 15-min preincubation were 3,5 and 0,65 μM, respectively. Experimental results obeyed a kinetic scheme, which supposed 2 extra substrate molecules bounding with the enzyme-substrate complex in the presence of linoleyl-hydroxamic acid.

Original languageEnglish (US)
Pages (from-to)36-37
Number of pages2
JournalUkrain'skyi Biokhimichnyi Zhurnal
Volume71
Issue number1
StatePublished - Jan 1 1999

ASJC Scopus subject areas

  • Biochemistry

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