Abstract
Linoleic acid oxidation by 12-lipoxygenase from porcine leukocytes has been studied as affected by linoleyl-hydroxamic acid. Linoleyl-hydroxamic acid has been found to be an effective inhibitor of porcine leucocyte 12-lipoxygenase. Aerobic preincubation of 12-lipoxygenase with 0, 1-6 μM of linoleyl-hydroxamic acid led to a time- and dose-dependent inhibition of the enzyme. The inhibitor's concentration able to induce a 50% loss of the enzyme activity with and without 15-min preincubation were 3,5 and 0,65 μM, respectively. Experimental results obeyed a kinetic scheme, which supposed 2 extra substrate molecules bounding with the enzyme-substrate complex in the presence of linoleyl-hydroxamic acid.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 36-37 |
| Number of pages | 2 |
| Journal | Ukrain'skyi Biokhimichnyi Zhurnal |
| Volume | 71 |
| Issue number | 1 |
| State | Published - Jan 1 1999 |
ASJC Scopus subject areas
- Biochemistry