Inhibition of myogenic differentiation by fibroblast growth factor or type β transforming growth factor does not require persistent c-myc expression

Gwendolyn Spizz, Jing Shan Hu, Eric N. Olson

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Skeletal muscle differentiation is accompanied by accumulation of the mRNA encoding the muscle isoenzyme of creatine kinase (MCK) and can be suppressed by serum components, fibroblast growth factor (FGF), or type β transforming growth factor (TGFβ). Using the nonfusing myogenic cell line, BC3H1, the potential involvement of c-myc in growth factor-dependent inhibition of myogenesis was examined. Withdrawal of undifferentiated myoblasts from the cell cycle in medium with 0.5% serum was associated with a precipitous decline in expression of c-myc mRNA followed by induction of MCK mRNA. In 0.5% serum containing TGFβ, c-myc mRNA declined to a level identical to that in differentiated cells; however, MCK mRNA was not expressed. Exposure of quiescent differentiated cells to FGF or TGFβ caused disappearance of muscle-specific gene products and was accompanied by only transient low level induction of c-myc mRNA. These data indicate that persistent c-myc expression is not required for growth factor-mediated inhibition of myogenic differentiation.

Original languageEnglish (US)
Pages (from-to)500-507
Number of pages8
JournalDevelopmental Biology
Volume123
Issue number2
DOIs
StatePublished - Oct 1987

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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