Abstract
Bone marrow stromal cells (BMSCs) can proliferate in vitro and can be transplanted for treating many kinds of diseases. However, BMSCs become senescent with long-term culture, which inhibits their application. To understand the mechanism underlying the senescence, we investigated the activity of phosphatidylcholine-specific phospholipase C (PC-PLC) and levels of integrin β4, caveolin-1 and ROS with BMSC senescence. The activity of PC-PLC and levels of integrin β4, caveolin-1 and ROS increased greatly during cell senescence. Selective inhibition of increased PC-PLC activity with D609 significantly decreased the number of senescence-associated beta galactosidase positive cells in BMSCs. Furthermore, D609 restored proliferation of BMSCs and their differentiation into adipocytes. Moreover, D609 suppressed the elevated levels of integrin β4, caveolin-1 and ROS. The data suggest that PC-PLC is involved in senescence of BMSCs, and its function is associated with integrin b4, caveolin-1 and ROS.
Original language | English (US) |
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Pages (from-to) | 519-528 |
Number of pages | 10 |
Journal | Journal of Cellular Biochemistry |
Volume | 108 |
Issue number | 2 |
DOIs | |
State | Published - Oct 1 2009 |
Externally published | Yes |
Keywords
- Bone marrow stromal cells
- Caveolin-1
- Integrin β4
- Phosphatidylcholine-specific phospholipase C
- ROS
- Senescence
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology