Inhibition of phosphatidylcholine-specific phospholipase C prevents bone marrow stromal cell senescence in vitro

Chunhui Sun, Nan Wang, Jie Huang, Jie Xin, Fen Peng, Yinshi Ren, Shangli Zhang, Junying Miao

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Bone marrow stromal cells (BMSCs) can proliferate in vitro and can be transplanted for treating many kinds of diseases. However, BMSCs become senescent with long-term culture, which inhibits their application. To understand the mechanism underlying the senescence, we investigated the activity of phosphatidylcholine-specific phospholipase C (PC-PLC) and levels of integrin β4, caveolin-1 and ROS with BMSC senescence. The activity of PC-PLC and levels of integrin β4, caveolin-1 and ROS increased greatly during cell senescence. Selective inhibition of increased PC-PLC activity with D609 significantly decreased the number of senescence-associated beta galactosidase positive cells in BMSCs. Furthermore, D609 restored proliferation of BMSCs and their differentiation into adipocytes. Moreover, D609 suppressed the elevated levels of integrin β4, caveolin-1 and ROS. The data suggest that PC-PLC is involved in senescence of BMSCs, and its function is associated with integrin b4, caveolin-1 and ROS.

Original languageEnglish (US)
Pages (from-to)519-528
Number of pages10
JournalJournal of Cellular Biochemistry
Volume108
Issue number2
DOIs
StatePublished - Oct 1 2009
Externally publishedYes

Keywords

  • Bone marrow stromal cells
  • Caveolin-1
  • Integrin β4
  • Phosphatidylcholine-specific phospholipase C
  • ROS
  • Senescence

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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