Inhibition of phosphatidylcholine-specific phospholipase C prevents bone marrow stromal cell senescence in vitro

Chunhui Sun, Nan Wang, Jie Huang, Jie Xin, Fen Peng, Yinshi Ren, Shangli Zhang, Junying Miao

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Bone marrow stromal cells (BMSCs) can proliferate in vitro and can be transplanted for treating many kinds of diseases. However, BMSCs become senescent with long-term culture, which inhibits their application. To understand the mechanism underlying the senescence, we investigated the activity of phosphatidylcholine-specific phospholipase C (PC-PLC) and levels of integrin β4, caveolin-1 and ROS with BMSC senescence. The activity of PC-PLC and levels of integrin β4, caveolin-1 and ROS increased greatly during cell senescence. Selective inhibition of increased PC-PLC activity with D609 significantly decreased the number of senescence-associated beta galactosidase positive cells in BMSCs. Furthermore, D609 restored proliferation of BMSCs and their differentiation into adipocytes. Moreover, D609 suppressed the elevated levels of integrin β4, caveolin-1 and ROS. The data suggest that PC-PLC is involved in senescence of BMSCs, and its function is associated with integrin b4, caveolin-1 and ROS.

Original languageEnglish (US)
Pages (from-to)519-528
Number of pages10
JournalJournal of Cellular Biochemistry
Volume108
Issue number2
DOIs
StatePublished - Oct 1 2009
Externally publishedYes

Keywords

  • Bone marrow stromal cells
  • Caveolin-1
  • Integrin β4
  • Phosphatidylcholine-specific phospholipase C
  • ROS
  • Senescence

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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