Inhibition of the ω-conotoxin-sensitive calcium current by distinct G proteins

R. Taussig, S. Sanchez, M. Rifo, A. G. Gilman, F. Belardetti

Research output: Contribution to journalArticle

107 Scopus citations

Abstract

Leu-enkephalin (Leu-Enk), norepinephrine (NE), somatostatin (SS), and bradykinin (BK) decrease the voltage-dependent calcium current in NG108-15 cells. Here we have investigated whether distinct G proteins, or a G protein common to all of the pathways, mediates this inhibition. We found that pertussis toxin (PTX) reduced all of these transmitter actions, except that of BK. To examine which of the PTX-sensitive pathways is transduced by GoA, we constructed an NG 108-15 cell line that stably expresses a mutant, PTX-resistant α subunit of GoA. After treatment with PTX, the mutant GoAα rescued the Leu-Enk and NE pathways but not the SS pathway. At least three different G proteins can transduce receptor-mediated inhibition of calcium currents in nerve cells. The effects of these G proteins appear to converge on the ω-conotoxin GVIA-sensitive calcium current.

Original languageEnglish (US)
Pages (from-to)799-809
Number of pages11
JournalNeuron
Volume8
Issue number4
DOIs
StatePublished - Apr 1992

ASJC Scopus subject areas

  • Neuroscience(all)

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