TY - JOUR
T1 - Inhibition of the ω-conotoxin-sensitive calcium current by distinct G proteins
AU - Taussig, R.
AU - Sanchez, S.
AU - Rifo, M.
AU - Gilman, A. G.
AU - Belardetti, F.
N1 - Funding Information:
We are indebted to R. Reed for providing the GoA,, cDNA, T. Higashijima for the experiment with the muscarinic receptors, S.Siegelbaumand F.JaramilloforcomputerprogramsandusefuI comments, M. Clark for expert technical assistance, D. Cali, and K. Edwards for excellent secretarial assistance. This work was supported by grants from the Klingenstein Fund and the U. S. Public Health Servicegrant NS25186(F. B.), by U. S. Public Health Service grant CM34497, American Cancer Society grant BE30M, the Raymond and Ellen Willie Chair of Molecular Neuropharmacology, the Lucille P. Markey Charitable Trust, and the Perot Family Foundation (A. G. C.). R. T. was supported by a Merck Postdoctoral Fellowship. F. B. is an Established Investigator of the American Heart Association.
PY - 1992/4
Y1 - 1992/4
N2 - Leu-enkephalin (Leu-Enk), norepinephrine (NE), somatostatin (SS), and bradykinin (BK) decrease the voltage-dependent calcium current in NG108-15 cells. Here we have investigated whether distinct G proteins, or a G protein common to all of the pathways, mediates this inhibition. We found that pertussis toxin (PTX) reduced all of these transmitter actions, except that of BK. To examine which of the PTX-sensitive pathways is transduced by GoA, we constructed an NG 108-15 cell line that stably expresses a mutant, PTX-resistant α subunit of GoA. After treatment with PTX, the mutant GoAα rescued the Leu-Enk and NE pathways but not the SS pathway. At least three different G proteins can transduce receptor-mediated inhibition of calcium currents in nerve cells. The effects of these G proteins appear to converge on the ω-conotoxin GVIA-sensitive calcium current.
AB - Leu-enkephalin (Leu-Enk), norepinephrine (NE), somatostatin (SS), and bradykinin (BK) decrease the voltage-dependent calcium current in NG108-15 cells. Here we have investigated whether distinct G proteins, or a G protein common to all of the pathways, mediates this inhibition. We found that pertussis toxin (PTX) reduced all of these transmitter actions, except that of BK. To examine which of the PTX-sensitive pathways is transduced by GoA, we constructed an NG 108-15 cell line that stably expresses a mutant, PTX-resistant α subunit of GoA. After treatment with PTX, the mutant GoAα rescued the Leu-Enk and NE pathways but not the SS pathway. At least three different G proteins can transduce receptor-mediated inhibition of calcium currents in nerve cells. The effects of these G proteins appear to converge on the ω-conotoxin GVIA-sensitive calcium current.
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U2 - 10.1016/0896-6273(92)90100-R
DO - 10.1016/0896-6273(92)90100-R
M3 - Article
C2 - 1348951
AN - SCOPUS:0026578284
SN - 0896-6273
VL - 8
SP - 799
EP - 809
JO - Neuron
JF - Neuron
IS - 4
ER -