Inhibition of transcription by bisPNA-peptide conjugates

Xin Zhao, Kunihiro Kaihatsu, David R. Corey

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Homopyrimidine bisPNAs have been reported to arrest transcription elongation by invading double-stranded DNA and forming a stable (PNA) 2/DNA complex. We previously reported that attachment of a designed cationic peptide to the bisPNA enhances the efficiency of strand invasion. Here we investigate whether conjugation to cationic peptides can also improve inhibition of transcription. We observe that a conjugate between a bisPNA and a peptide containing eight lysines is a superior agent for inhibition of transcription, but that inhibition of transcription is reduced as pH and the concentration of magnesium are increased. Our studies provide useful characterization of bisPNAs as agents for inhibiting transcription.

Original languageEnglish (US)
Pages (from-to)535-546
Number of pages12
JournalNucleosides, Nucleotides and Nucleic Acids
Volume22
Issue number5-8
DOIs
StatePublished - Sep 29 2003

Keywords

  • PNA
  • Peptide
  • Peptide nucleic acid
  • Transcription inhibition

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Genetics

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