Initial attachment of baby hamster kidney cells to an epoxy substratum: ultrastructural analysis

F. Grinnell, M. Q. Tobleman, C. R. Hackenbrock

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In the presence of serum containing medium, BHK cells attached and spread during a 1 hr period onto a 3 to 5 nm thick serum layer adsorbed on the substratum surface. The closest approach of the plasma membrane to the serum layer was observed to be about 9 nm, which was determined by tilting the sectioned cells in a goniometer holder. Bundles of microfilaments or other cytoplasmic specializations were not observed in association with the regions of close contact. However, in the space between the plasma membrane and the adsorbed serum layer, a diffusely stained material could be visualized after fixation/staining by the tannic acid glutaraldehyde technique. This technique also permitted increased clarity of visualization of the trilaminar appearance of the plasma membrane. The distribution and mobility of anionic sites on the surfaces of attached and spreading cells was determined by labeling with polycationic ferritin. The authors observed movement of polycationic ferritin into large clusters on the cell surface, collapse of cell surface microextensions, and endocytosis, all of which were similar to the previous findings utilizing cells in suspension. However, the absolute amount of ferritin bound to the upper cell surface was less than that previously observed when suspended cells were put under similar labeling conditions. Also, polycationic ferritin did not appear to penetrate between the lower cell surface and the substratum.

Original languageEnglish (US)
Pages (from-to)707-713
Number of pages7
JournalJournal of Cell Biology
Volume70
Issue number3
StatePublished - 1976

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Cricetinae
Kidney
Cell Membrane
Serum
Tannins
Glutaral
Ferritins
Endocytosis
Actin Cytoskeleton
Suspensions
Staining and Labeling
polycationic ferritin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Initial attachment of baby hamster kidney cells to an epoxy substratum : ultrastructural analysis. / Grinnell, F.; Tobleman, M. Q.; Hackenbrock, C. R.

In: Journal of Cell Biology, Vol. 70, No. 3, 1976, p. 707-713.

Research output: Contribution to journalArticle

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