Initiation of human parturition. VI. Identification and quantification of progesterone metabolites produced by the components of human fetal membranes

L. Milewich, N. F. Gant, B. E. Schwarz, R. A. Prough, G. T. Chen, B. Athey, P. C. Macdonald

Research output: Contribution to journalArticle

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Abstract

The fetal membranes play a central role in the initiation of human parturition, a role that may be subserved in part by quantitative changes in progesterone metabolism. In order to identify and to quantify the metabolites of progesterone produced by the components of the human fetal membranes, amnion and chorion laeve tissues and homogenates were incubated with [ 3H]progesterone in the presence of added NADPH. The radioactive metabolites, 20α-hydroxy-4-pregnen-3-one, 5α-pregnane-3,20-dione and 3β-hydroxy-5α-pregnan-20-one were isolated and identified by derivative formation, a combination of chromatographic techniques, and by crystallization to constant specific activity after addition of authentic standards. A simplified technique of metabolite quantification was developed that involves one thin layer chromatographic procedure and liquid scintillation assay of radioactivity. The accuracy of this method was established by comparison with data obtained using classical techniques. This study demonstrated the presence of human fetal membranes 5α-reductase, 20α-hydroxysteroid oxidoreductase, and 3β-hydroxysteroid oxidoreductase, and a qualitative difference between amnion and chorion laeve.

Original languageEnglish (US)
Pages (from-to)400-411
Number of pages12
JournalJournal of Clinical Endocrinology and Metabolism
Volume45
Issue number3
StatePublished - 1977

Fingerprint

Extraembryonic Membranes
Metabolites
Hydroxysteroids
Progesterone
Chorion
Oxidoreductases
Amnion
Parturition
Membranes
Pregnanes
Radioactivity
Scintillation
Crystallization
NADP
Metabolism
Assays
Tissue
Derivatives
Liquids

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Initiation of human parturition. VI. Identification and quantification of progesterone metabolites produced by the components of human fetal membranes. / Milewich, L.; Gant, N. F.; Schwarz, B. E.; Prough, R. A.; Chen, G. T.; Athey, B.; Macdonald, P. C.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 45, No. 3, 1977, p. 400-411.

Research output: Contribution to journalArticle

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AU - Gant, N. F.

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AU - Prough, R. A.

AU - Chen, G. T.

AU - Athey, B.

AU - Macdonald, P. C.

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