Injury enhances resistance to Escherichia coli infection by boosting innate immune system function

Adrian A. Maung, Satoshi Fujimi, Malcolm P. MacConmara, Goro Tajima, Ann M. McKenna, Adam J. Delisle, Christopher Stallwood, Andrew B. Onderdonk, John A. Mannick, James A. Lederer

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Major injury is widely thought to predispose the injured host to opportunistic infections. This idea is supported by animal studies showing that major injury causes reduced resistance to polymicrobial sepsis induced by cecal ligation and puncture. Although cecal ligation and puncture represents a clinically relevant sepsis model, we wanted to test whether injury might also lead to greater susceptibility to peritoneal infection caused by a single common pathogen, Escherichia coli. Contrary to our expectation, we show herein that the LD50 for sham-injured mice was 103 CFU of E. coli, whereas the LD50 for burn-injured mice was 50 × 103 CFU at 7 days postinjury. This injury-associated enhanced resistance was apparent as early as 1 day after injury, and maximal resistance was observed at days 7 and 14. We found that burn-injured mice had higher numbers of circulating neutrophils and monocytes than did sham mice before infection and that injured mice were able to recruit greater numbers of neutrophils to the site of infection. Moreover, the peritoneal neutrophils in burn-injured mice were more highly activated than neutrophils from sham mice as determined by Mac-1 expression, superoxide generation, and bactericidal activity. Our findings suggest that the enhanced innate immune response that develops following injury, although it is commonly accepted as the mediator of the detrimental systemic inflammatory response syndrome, may also, in some cases, benefit the injured host by boosting innate immune antimicrobial defenses.

Original languageEnglish (US)
Pages (from-to)2450-2458
Number of pages9
JournalJournal of Immunology
Volume180
Issue number4
StatePublished - Feb 15 2008

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Escherichia coli Infections
Immune System
Wounds and Injuries
Neutrophils
Burns
Lethal Dose 50
Punctures
Ligation
Sepsis
Infection
Escherichia coli
Systemic Inflammatory Response Syndrome
Erythroid Precursor Cells
Opportunistic Infections
Innate Immunity
Superoxides
Monocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Maung, A. A., Fujimi, S., MacConmara, M. P., Tajima, G., McKenna, A. M., Delisle, A. J., ... Lederer, J. A. (2008). Injury enhances resistance to Escherichia coli infection by boosting innate immune system function. Journal of Immunology, 180(4), 2450-2458.

Injury enhances resistance to Escherichia coli infection by boosting innate immune system function. / Maung, Adrian A.; Fujimi, Satoshi; MacConmara, Malcolm P.; Tajima, Goro; McKenna, Ann M.; Delisle, Adam J.; Stallwood, Christopher; Onderdonk, Andrew B.; Mannick, John A.; Lederer, James A.

In: Journal of Immunology, Vol. 180, No. 4, 15.02.2008, p. 2450-2458.

Research output: Contribution to journalArticle

Maung, AA, Fujimi, S, MacConmara, MP, Tajima, G, McKenna, AM, Delisle, AJ, Stallwood, C, Onderdonk, AB, Mannick, JA & Lederer, JA 2008, 'Injury enhances resistance to Escherichia coli infection by boosting innate immune system function', Journal of Immunology, vol. 180, no. 4, pp. 2450-2458.
Maung AA, Fujimi S, MacConmara MP, Tajima G, McKenna AM, Delisle AJ et al. Injury enhances resistance to Escherichia coli infection by boosting innate immune system function. Journal of Immunology. 2008 Feb 15;180(4):2450-2458.
Maung, Adrian A. ; Fujimi, Satoshi ; MacConmara, Malcolm P. ; Tajima, Goro ; McKenna, Ann M. ; Delisle, Adam J. ; Stallwood, Christopher ; Onderdonk, Andrew B. ; Mannick, John A. ; Lederer, James A. / Injury enhances resistance to Escherichia coli infection by boosting innate immune system function. In: Journal of Immunology. 2008 ; Vol. 180, No. 4. pp. 2450-2458.
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