After reading the paper by Gazdar and Dammin in the Journal (283:222–224, 1970), I believe a few comments regarding technic are in order. The authors, in their excellent study of human renal neural degeneration and regeneration after transplantation, used a modified Bodian silver stain. Unfortunately, they did not make use of either fluorescence histochemistry or histochemical staining of acetylcholinesterase, which sensitively and specifically demonstrate adrenergic and cholinergic neurons. The authors used autopsy specimens on which these technics cannot be applied. With these histochemical methods Norvell and I1,2 found neuronal degeneration much sooner than Gazdar and Dammin did;.
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