Inositol phospholipid metabolism may trigger flagellar excision in Chlamydomonas reinhardtii

L. M. Quarmby, Y. G. Yueh, J. L. Cheshire, L. R. Keller, W. J. Snell, R. C. Crain

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Chlamydomonas reinhardtii cells shed their flagella in response to environmental stress. Under favorable conditions, flagella are quickly regrown. To learn more about the signals that trigger flagellar excision and regrowth we have investigated inositol phospholipid metabolites, molecules implicated in signal transduction in several other systems. After deflagellation by low pH or mastoparan, a potent activator of G proteins, there was a rapid increase in levels of inositol 1,4,5-trisphosphate measured by use of receptor-binding assays and HPLC. This increase was concomitant with a decrease in levels of phosphatidylinositol 4,5-bisphosphate and was followed by an increase in phosphatidic acid, results consistent with activation of phospholipase C and diacylglycerol kinase. Additional experiments suggest that this activated phospholipase C is not important for flagellar regrowth but plays a role in informing the excision apparatus of the environmental stress. Addition of neomycin (an inhibitor of phospholipase C) before exposure of cells to low pH or mastoparan prevented the increase in inositol 1,4,5-trisphosphate and also prevented deflagellation. Addition of neomycin after deflagellation blocked increases in inositol 1,4,5-trisphosphate that normally followed deflagellation, but did not block flagellar assembly. Furthermore, a flagellar excision-defective mutant, fa-1, did not shed its flagella in response to low pH or mastoparan, yet both of these agents activated phospholipase C in these cells. The results suggest that activation of phospholipase C, possibly via a G protein, is a proximal step in the signal transduction pathway inducing deflagellation in Chlamydomonas.

Original languageEnglish (US)
Pages (from-to)737-744
Number of pages8
JournalJournal of Cell Biology
Volume116
Issue number3
StatePublished - Feb 1992

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Chlamydomonas reinhardtii
Type C Phospholipases
Phosphatidylinositols
Inositol 1,4,5-Trisphosphate
Flagella
Neomycin
GTP-Binding Proteins
Signal Transduction
Diacylglycerol Kinase
Chlamydomonas
Phosphatidic Acids
High Pressure Liquid Chromatography
mastoparan

ASJC Scopus subject areas

  • Cell Biology

Cite this

Quarmby, L. M., Yueh, Y. G., Cheshire, J. L., Keller, L. R., Snell, W. J., & Crain, R. C. (1992). Inositol phospholipid metabolism may trigger flagellar excision in Chlamydomonas reinhardtii. Journal of Cell Biology, 116(3), 737-744.

Inositol phospholipid metabolism may trigger flagellar excision in Chlamydomonas reinhardtii. / Quarmby, L. M.; Yueh, Y. G.; Cheshire, J. L.; Keller, L. R.; Snell, W. J.; Crain, R. C.

In: Journal of Cell Biology, Vol. 116, No. 3, 02.1992, p. 737-744.

Research output: Contribution to journalArticle

Quarmby, LM, Yueh, YG, Cheshire, JL, Keller, LR, Snell, WJ & Crain, RC 1992, 'Inositol phospholipid metabolism may trigger flagellar excision in Chlamydomonas reinhardtii', Journal of Cell Biology, vol. 116, no. 3, pp. 737-744.
Quarmby, L. M. ; Yueh, Y. G. ; Cheshire, J. L. ; Keller, L. R. ; Snell, W. J. ; Crain, R. C. / Inositol phospholipid metabolism may trigger flagellar excision in Chlamydomonas reinhardtii. In: Journal of Cell Biology. 1992 ; Vol. 116, No. 3. pp. 737-744.
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