Inositol trisphosphate is required for the propagation of calcium waves in Xenopus oocytes

S. DeLisle, M. J. Welsh

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Stimuli which act through the second messenger inositol 1,4,5- trisphosphate (InsP3) often increase free intracellular Ca2+ concentration ([Ca2+](i)) in a localized subcellular area. Actively propagated Ca2+ waves then extend this focal Ca2+ signal to other parts of the cell. To understand how cells may control the spatial distribution of Ca2+, we investigated the mechanism by which Ca2+ waves propagate through the cytoplasm of Xenopus oocytes. Heparin, which inhibits the binding of InsP3 to its receptor, prevented the migration of Ca2+ waves induced by a poorly metabolized InsP3 (InsP3S3). This result suggested that Ca2+ waves move through the cell via the serial release of Ca2+ from InsP3-sensitive stores. Interventions which caused a localized increase in [Ca2+](i) without elevations of InsP3 did not trigger Ca2+ waves. In the presence of a InsP3S3, however, endogenously released or locally injected Ca2+ elicited Ca2+ waves. A cooperative interaction between Ca2+ and InsP3 may therefore be responsible for the propagation of Ca2+ waves.

Original languageEnglish (US)
Pages (from-to)7963-7966
Number of pages4
JournalJournal of Biological Chemistry
Volume267
Issue number12
StatePublished - Jan 1 1992

Fingerprint

Calcium Signaling
Inositol
Xenopus
Oocytes
Calcium
Inositol 1,4,5-Trisphosphate
Second Messenger Systems
Heparin
Cytoplasm
Spatial distribution

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Inositol trisphosphate is required for the propagation of calcium waves in Xenopus oocytes. / DeLisle, S.; Welsh, M. J.

In: Journal of Biological Chemistry, Vol. 267, No. 12, 01.01.1992, p. 7963-7966.

Research output: Contribution to journalArticle

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