Insights into pilus assembly and secretion from the structure and functional characterization of usher PapC

Yihua Huang, Barbara S. Smith, Lucy X. Chen, Richard H G Baxter, Johann Deisenhofer

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Ushers constitute a family of bacterial outer membrane proteins responsible for the assembly and secretion of surface organelles such as the pilus. The structure at 3.15-Å resolution of the usher pyelonephritis-associated pili C (PapC) translocation domain reveals a 24-stranded kidney-shaped β-barrel, occluded by an internal plug domain. The dimension of the pore allows tandem passage of individual folded pilus subunits in an upright pilus growth orientation, but is insufficient for accommodating donor strand exchange. The molecular packing revealed by the crystal structure shows that 2 PapC molecules in head-to-head orientation interact via exposed β-strand edges, which could be the preferred dimer interaction in solution. In vitro reconstitution of fiber assemblies suggest that PapC monomers may be sufficient for fiber assembly and secretion; both the plug domain and the C-terminal domain of PapC are required for filament assembly, whereas the N-terminal domain is mainly responsible for recruiting the chap- erone-subunit complexes to the usher. The plug domain has a dual function: gating the β-pore and participating in pilus assembly.

Original languageEnglish (US)
Pages (from-to)7403-7407
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number18
DOIs
StatePublished - May 5 2009

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Pyelonephritis
Bacterial Outer Membrane Proteins
Organelles
Kidney
Growth

Keywords

  • Bacterial outer membrane protein
  • Crystal structure
  • In vitro pilus assembly

ASJC Scopus subject areas

  • General

Cite this

Insights into pilus assembly and secretion from the structure and functional characterization of usher PapC. / Huang, Yihua; Smith, Barbara S.; Chen, Lucy X.; Baxter, Richard H G; Deisenhofer, Johann.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 18, 05.05.2009, p. 7403-7407.

Research output: Contribution to journalArticle

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