Insulin, Glucagon, and Somatostatin Secretion by Cultured Rat Islet Cell Tumor and Its Clones

S. J. Bhathena, S. Awoke, N. R. Voyles, S. D. Wilkins, L. Recant, H. K. Oie, A. F. Gazdar

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Cells derived from rat islet tumor and grown in culture (parent cells—RIN-m) and two clones obtained from them were used to study the effect of various secretagogues on insulin, glucagon, and somatostatin secretion. Parent cells secreted all three hormones in various quantities, while clone 5F secreted predominantly insulin and clone 14B secreted predominantly somatostatin. The secretory behavior of these cells were compared to each other and to that of normal islets. In general, as in the case of normal islets, insulin secretion was stimulated by calcium, potassium, tolbutamide, theophylline, and glucagon. It was inhibited by somatostatin. Glucagon secretion was stimulated by calcium, arginine, and theophylline. Somatostatin secretion was stimulated in clone 14B by arginine, tolbutamide, theophylline, and insulin. These cells differ from normal islets, in that they do not respond to glucose or arginine with increased insulin secretion. Also somatostatin failed to inhibit glucagon secretion. The similarity in insulin secretory responses of parent cells and clone 5F suggests that local or paracrine islet hormone secretion plays only a negligible role in the control of other hormone secretion in these cells.

Original languageEnglish (US)
Pages (from-to)35-38
Number of pages4
JournalProceedings of the Society for Experimental Biology and Medicine
Volume175
Issue number1
DOIs
StatePublished - Jan 1984

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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