Insulin-like growth factor binding protein-3 secretion from cultured rat sertoli cells: Dual regulation by follicle stimulating hormone and insulin-like growth factor-I

Eric P. Smith, Bryan A. Dickson, Steven D. Chernausek

Research output: Contribution to journalArticle

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Abstract

The insulin-like growth factors (IGFs) are found in extracellular fluids bound to carrier proteins which influence the biological activity of the IGFs. Three structurally different binding proteins (BPs) have been isolated and cloned; each has distinct tissue specific expression and unique properties. We report here that testicular cells synthesize a specific subset of these binding proteins. Ligand blot analysis and RNA blot hybridization indicates that cultured peritubular cells synthesize primarily IGFBP-2. In contrast, as determined by ligand blot, RNA blot hybridization and N-linked deglycosylated studies, IGFBP-3 is predominantly synthesized by the Sertoli cell. In a dose dependent fashion, FSH markedly reduces the levels of IGFBP-3 in Sertoli cell conditioned medium. Similarly, isoproterenol, (Bu)2cAMP and cholera toxin also markedly reduce the abundance of IGFBP-3 in conditioned media. In contrast, IGF-I increases the concentrations of IGFBP-3 with the concentration required for half-maximal stimulation, approximately 20 ng/ml. Consistent with a peritubular cell origin, IGFBP-2 may be the predominant species found in interstitial fluid. In summary, our data reveal that the IGFBPs are expressed in a cell type specific manner in the testis. The opposing effects of FSH and IGF-I on Sertoli cell IGFBP-3 expression suggests a mechanism by which the IGF-I biological activity on Sertoli cell might be influenced.

Original languageEnglish (US)
Pages (from-to)2744-2751
Number of pages8
JournalEndocrinology
Volume127
Issue number6
StatePublished - Dec 1990

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Insulin-Like Growth Factor Binding Protein 3
Sertoli Cells
Follicle Stimulating Hormone
Insulin-Like Growth Factor I
Insulin-Like Growth Factor Binding Protein 2
Carrier Proteins
Extracellular Fluid
Somatomedins
Conditioned Culture Medium
RNA
Ligands
Insulin-Like Growth Factor Binding Proteins
Cholera Toxin
Isoproterenol
Testis
Cultured Cells

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Insulin-like growth factor binding protein-3 secretion from cultured rat sertoli cells : Dual regulation by follicle stimulating hormone and insulin-like growth factor-I. / Smith, Eric P.; Dickson, Bryan A.; Chernausek, Steven D.

In: Endocrinology, Vol. 127, No. 6, 12.1990, p. 2744-2751.

Research output: Contribution to journalArticle

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