Intact p53-dependent responses in miR-34-deficient mice

Carla P. Concepcion, Yoon Chi Han, Ping Mu, Ciro Bonetti, Evelyn Yao, Aleco D'Andrea, Joana A. Vidigal, William P. Maughan, Paul Ogrodowski, Andrea Ventura

Research output: Contribution to journalArticlepeer-review

138 Scopus citations

Abstract

MicroRNAs belonging to the miR-34 family have been proposed as critical modulators of the p53 pathway and potential tumor suppressors in human cancers. To formally test these hypotheses, we have generated mice carrying targeted deletion of all three members of this microRNA family. We show that complete inactivation of miR-34 function is compatible with normal development in mice. Surprisingly, p53 function appears to be intact in miR-34-deficient cells and tissues. Although loss of miR-34 expression leads to a slight increase in cellular proliferation in vitro, it does not impair p53-induced cell cycle arrest or apoptosis. Furthermore, in contrast to p53-deficient mice, miR-34-deficient animals do not display increased susceptibility to spontaneous, irradiation-induced, or c-Myc-initiated tumorigenesis. We also show that expression of members of the miR-34 family is particularly high in the testes, lungs, and brains of mice and that it is largely p53-independent in these tissues. These findings indicate that miR-34 plays a redundant function in the p53 pathway and suggest additional p53-independent functions for this family of miRNAs.

Original languageEnglish (US)
Article numbere1002797
JournalPLoS genetics
Volume8
Issue number7
DOIs
StatePublished - Jul 2012

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Fingerprint Dive into the research topics of 'Intact p53-dependent responses in miR-34-deficient mice'. Together they form a unique fingerprint.

Cite this