Integrated Metagenomics/Metaproteomics Reveals Human Host-Microbiota Signatures of Crohn's Disease

Alison R. Erickson, Brandi L. Cantarel, Regina Lamendella, Youssef Darzi, Emmanuel F. Mongodin, Chongle Pan, Manesh Shah, Jonas Halfvarson, Curt Tysk, Bernard Henrissat, Jeroen Raes, Nathan C. Verberkmoes, Claire M. Fraser, Robert L. Hettich, Janet K. Jansson

Research output: Contribution to journalArticle

240 Citations (Scopus)

Abstract

Crohn's disease (CD) is an inflammatory bowel disease of complex etiology, although dysbiosis of the gut microbiota has been implicated in chronic immune-mediated inflammation associated with CD. Here we combined shotgun metagenomic and metaproteomic approaches to identify potential functional signatures of CD in stool samples from six twin pairs that were either healthy, or that had CD in the ileum (ICD) or colon (CCD). Integration of these omics approaches revealed several genes, proteins, and pathways that primarily differentiated ICD from healthy subjects, including depletion of many proteins in ICD. In addition, the ICD phenotype was associated with alterations in bacterial carbohydrate metabolism, bacterial-host interactions, as well as human host-secreted enzymes. This eco-systems biology approach underscores the link between the gut microbiota and functional alterations in the pathophysiology of Crohn's disease and aids in identification of novel diagnostic targets and disease specific biomarkers.

Original languageEnglish (US)
Article numbere49138
JournalPLoS One
Volume7
Issue number11
DOIs
StatePublished - Nov 28 2012

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Metagenomics
Crohn disease
Microbiota
Crohn Disease
intestinal microorganisms
Dysbiosis
Systems Biology
inflammatory bowel disease
Firearms
Carbohydrate Metabolism
carbohydrate metabolism
pathophysiology
Ileum
Inflammatory Bowel Diseases
ileum
colon
etiology
biomarkers
Healthy Volunteers
Colon

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Erickson, A. R., Cantarel, B. L., Lamendella, R., Darzi, Y., Mongodin, E. F., Pan, C., ... Jansson, J. K. (2012). Integrated Metagenomics/Metaproteomics Reveals Human Host-Microbiota Signatures of Crohn's Disease. PLoS One, 7(11), [e49138]. https://doi.org/10.1371/journal.pone.0049138

Integrated Metagenomics/Metaproteomics Reveals Human Host-Microbiota Signatures of Crohn's Disease. / Erickson, Alison R.; Cantarel, Brandi L.; Lamendella, Regina; Darzi, Youssef; Mongodin, Emmanuel F.; Pan, Chongle; Shah, Manesh; Halfvarson, Jonas; Tysk, Curt; Henrissat, Bernard; Raes, Jeroen; Verberkmoes, Nathan C.; Fraser, Claire M.; Hettich, Robert L.; Jansson, Janet K.

In: PLoS One, Vol. 7, No. 11, e49138, 28.11.2012.

Research output: Contribution to journalArticle

Erickson, AR, Cantarel, BL, Lamendella, R, Darzi, Y, Mongodin, EF, Pan, C, Shah, M, Halfvarson, J, Tysk, C, Henrissat, B, Raes, J, Verberkmoes, NC, Fraser, CM, Hettich, RL & Jansson, JK 2012, 'Integrated Metagenomics/Metaproteomics Reveals Human Host-Microbiota Signatures of Crohn's Disease', PLoS One, vol. 7, no. 11, e49138. https://doi.org/10.1371/journal.pone.0049138
Erickson, Alison R. ; Cantarel, Brandi L. ; Lamendella, Regina ; Darzi, Youssef ; Mongodin, Emmanuel F. ; Pan, Chongle ; Shah, Manesh ; Halfvarson, Jonas ; Tysk, Curt ; Henrissat, Bernard ; Raes, Jeroen ; Verberkmoes, Nathan C. ; Fraser, Claire M. ; Hettich, Robert L. ; Jansson, Janet K. / Integrated Metagenomics/Metaproteomics Reveals Human Host-Microbiota Signatures of Crohn's Disease. In: PLoS One. 2012 ; Vol. 7, No. 11.
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