TY - JOUR
T1 - Integrative miRNA and whole-genome analyses of epicardial adipose tissue in patients with coronary atherosclerosis
AU - Vacca, Michele
AU - Di Eusanio, Marco
AU - Cariello, Marica
AU - Graziano, Giusi
AU - D'Amore, Simona
AU - Petridis, Francesco Dimitri
AU - D'Orazio, Andria
AU - Salvatore, Lorena
AU - Tamburro, Antonio
AU - Folesani, Gianluca
AU - Rutigliano, David
AU - Pellegrini, Fabio
AU - Sabbà, Carlo
AU - Palasciano, Giuseppe
AU - Di Bartolomeo, Roberto
AU - Moschetta, Antonio
N1 - Funding Information:
The authors are indebted to P. Caldarola, M. Copetti, N. Locuratolo, R. Mariani-Costantini, R. Salvia, L. Sublimi Saponetti and all the clinicians and nurses of Policlinico ''Malpighi'' of Bologna and of the ''San Paolo'' Hospital of Bari for criticisms and help during the study. The work was funded by NR-NET FP7 Marie Curie ITN (to A.M.), Italian Ministry of University and Education (Finanziamenti per la Ricerca di Base IDEAS RBID08C9N7 to A.M.; Programma Operativo Nazionale PON01_01958 to A.M.; PRIN 2010FHH32M-002 to A.M.; PRIN 20085Y7XT5_004 to G.P.); Italian Ministry of Health (Young Researchers Grants GR-2008-1143546 and GR-2010-2314703 to A.M.); Apulian Region (POR Strategic Projects, CIP PS_101 to G.P.); University of Bari, Italy (ORBA 08WEZJ, 07X7Q1, 06BXVC, IDEA GRBA0802SJ to A.M.). M.V. is supported by the Fondazione ''Umberto Veronesi'' (fellowship) and by the Medical Research Council (MRC-HNR; MC_PC_13030). M.C. is a postdoctoral fellow of AIRC.
Publisher Copyright:
© 2015 Published on behalf of the European Society of Cardiology. All rights reserved The Author 2015.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Background Epicardial adipose tissue (EAT) is an atypical fat depot surrounding the heart with a putative role in the development of atherosclerosis. Methods and results We profiled genes and miRNAs in perivascular EAT and subcutaneous adipose tissue (SAT) of metabolically healthy patients without coronary artery disease (CAD) vs. metabolic patients with CAD. Compared with SAT, a specific tuning of miRNAs and genes points to EAT as a tissue characterized by a metabolically active and pro-inflammatory profile. Then, we depicted both miRNA and gene signatures of EAT in CAD, featuring a down-regulation of genes involved in lipid metabolism, mitochondrial function, nuclear receptor transcriptional activity, and an up-regulation of those involved in antigen presentation, chemokine signalling, and inflammation. Finally, we identified miR-103-3p as candidate modulator of CCL13 in EAT, and a potential biomarker role for the chemokine CCL13 in CAD. Conclusion EAT in CAD is characterized by changes in the regulation of metabolism and inflammation with miR-103-3p/CCL13 pair as novel putative actors in EAT function and CAD.
AB - Background Epicardial adipose tissue (EAT) is an atypical fat depot surrounding the heart with a putative role in the development of atherosclerosis. Methods and results We profiled genes and miRNAs in perivascular EAT and subcutaneous adipose tissue (SAT) of metabolically healthy patients without coronary artery disease (CAD) vs. metabolic patients with CAD. Compared with SAT, a specific tuning of miRNAs and genes points to EAT as a tissue characterized by a metabolically active and pro-inflammatory profile. Then, we depicted both miRNA and gene signatures of EAT in CAD, featuring a down-regulation of genes involved in lipid metabolism, mitochondrial function, nuclear receptor transcriptional activity, and an up-regulation of those involved in antigen presentation, chemokine signalling, and inflammation. Finally, we identified miR-103-3p as candidate modulator of CCL13 in EAT, and a potential biomarker role for the chemokine CCL13 in CAD. Conclusion EAT in CAD is characterized by changes in the regulation of metabolism and inflammation with miR-103-3p/CCL13 pair as novel putative actors in EAT function and CAD.
KW - Epicardial adipose tissue
KW - Gene expression
KW - Metabolic syndrome
KW - Nuclear receptors
KW - miRNA
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U2 - 10.1093/cvr/cvv266
DO - 10.1093/cvr/cvv266
M3 - Article
C2 - 26645979
AN - SCOPUS:84959867280
VL - 109
SP - 228
EP - 239
JO - Cardiovascular Research
JF - Cardiovascular Research
SN - 0008-6363
IS - 2
ER -