TY - JOUR
T1 - Integrin αIIbβ3 induces the adhesion and activation of mast cells through interaction with fibrinogen
AU - Oki, Toshihiko
AU - Kitaura, Jiro
AU - Eto, Koji
AU - Lu, Yang
AU - Maeda-Yamamoto, Mari
AU - Inagaki, Naoki
AU - Nagai, Hiroichi
AU - Yamanishi, Yoshinori
AU - Nakajina, Hideaki
AU - Kumagai, Hidetoshi
AU - Kitamura, Toshio
PY - 2006/1/1
Y1 - 2006/1/1
N2 - Integrin αIIb, a well-known marker of megakaryocyte- platelet lineage, has been recently recognized on hemopoietic progenitors. We now demonstrate that integrin αIIbβ3 is highly expressed on mouse and human mast cells including mouse bone marrow-derived mast cells, peritoneal mast cells, and human cord blood-derived mast cells, and that its binding to extracellular matrix proteins leads to enhancement of biological functions of mast cells in concert with various stimuli. With exposure to various stimuli, including cross-linking of FcεcRI and stem cell factor, mast cells adhered to extracellular matrix proteins such as fibrinogen and von Willebrand factor in an integrin αIIbβ3- dependent manner. In addition, the binding of mast cells to fibrinogen enhanced proliferation, cytokine production, and migration and induced uptake of soluble fibrinogen in response to stem cell factor stimulation, implicating integrin αIIbβ3 in a variety of mast cell functions. In conclusion, mouse and human mast cells express functional integrin αIIbβ3.
AB - Integrin αIIb, a well-known marker of megakaryocyte- platelet lineage, has been recently recognized on hemopoietic progenitors. We now demonstrate that integrin αIIbβ3 is highly expressed on mouse and human mast cells including mouse bone marrow-derived mast cells, peritoneal mast cells, and human cord blood-derived mast cells, and that its binding to extracellular matrix proteins leads to enhancement of biological functions of mast cells in concert with various stimuli. With exposure to various stimuli, including cross-linking of FcεcRI and stem cell factor, mast cells adhered to extracellular matrix proteins such as fibrinogen and von Willebrand factor in an integrin αIIbβ3- dependent manner. In addition, the binding of mast cells to fibrinogen enhanced proliferation, cytokine production, and migration and induced uptake of soluble fibrinogen in response to stem cell factor stimulation, implicating integrin αIIbβ3 in a variety of mast cell functions. In conclusion, mouse and human mast cells express functional integrin αIIbβ3.
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U2 - 10.4049/jimmunol.176.1.52
DO - 10.4049/jimmunol.176.1.52
M3 - Article
C2 - 16365395
AN - SCOPUS:29644437920
SN - 0022-1767
VL - 176
SP - 52
EP - 60
JO - Journal of Immunology
JF - Journal of Immunology
IS - 1
ER -