Integrin-dependent actomyosin contraction regulates epithelial cell scattering

Johan De Rooij, Andre Kerstens, Gaudenz Danuser, Martin A. Schwartz, Clare M. Waterman-Storer

Research output: Contribution to journalArticle

218 Citations (Scopus)

Abstract

The scattering of Madin-Darby canine kidney cells in vitro mimics key aspects of epithelial-mesenchymal transitions during development, carcinoma cell invasion, and metastasis. Scattering is induced by hepatocyte growth factor (HGF) and is thought to involve disruption of cadherin-dependent cell-cell junctions. Scattering is enhanced on collagen and fibronectin, as compared with laminin1, suggesting possible cross talk between integrins and cell-cell junctions. We show that HGF does not trigger any detectable decrease in E-cadherin function, but increases integrin-mediated adhesion. Time-lapse imaging suggests that tension on cell-cell junctions may disrupt cell-cell adhesion. Varying the density and type of extracellular matrix proteins shows that scattering correlates with stronger integrin adhesion and increased phosphorylation of the myosin regulatory light chain. To directly test the role of integrin-dependent traction forces, substrate compliance was varied. Rigid substrates that produce high traction forces promoted scattering, in comparison to more compliant substrates. We conclude that integrindependent actomyosin traction force mediates the disruption of cell-cell adhesion during epithelial cell scattering.

Original languageEnglish (US)
Pages (from-to)153-164
Number of pages12
JournalJournal of Cell Biology
Volume171
Issue number1
DOIs
StatePublished - Oct 2005

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Actomyosin
Integrins
Epithelial Cells
Intercellular Junctions
Traction
Hepatocyte Growth Factor
Cadherins
Cell Adhesion
Time-Lapse Imaging
Myosin Light Chains
Madin Darby Canine Kidney Cells
Epithelial-Mesenchymal Transition
Extracellular Matrix Proteins
Fibronectins
Compliance
Collagen
Phosphorylation
Neoplasm Metastasis
Carcinoma

ASJC Scopus subject areas

  • Cell Biology

Cite this

Integrin-dependent actomyosin contraction regulates epithelial cell scattering. / De Rooij, Johan; Kerstens, Andre; Danuser, Gaudenz; Schwartz, Martin A.; Waterman-Storer, Clare M.

In: Journal of Cell Biology, Vol. 171, No. 1, 10.2005, p. 153-164.

Research output: Contribution to journalArticle

De Rooij, J, Kerstens, A, Danuser, G, Schwartz, MA & Waterman-Storer, CM 2005, 'Integrin-dependent actomyosin contraction regulates epithelial cell scattering', Journal of Cell Biology, vol. 171, no. 1, pp. 153-164. https://doi.org/10.1083/jcb.200506152
De Rooij, Johan ; Kerstens, Andre ; Danuser, Gaudenz ; Schwartz, Martin A. ; Waterman-Storer, Clare M. / Integrin-dependent actomyosin contraction regulates epithelial cell scattering. In: Journal of Cell Biology. 2005 ; Vol. 171, No. 1. pp. 153-164.
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