Inter-tissue and inter-species characteristics of the mitochondrial carnitine palmitoyltransferase enzyme system

Keith F. Woeltje, Victoria Esser, Brian C. Weis, William F. Cox, James G. Schroeder, Shyue Tsong Liao, Daniel W. Foster, J. Denis McGarry

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Abstract

Properties of the carnitine palmitoyltransferase (EC 2.3.1.21) (CPT) enzyme system were compared in isolated mitochondria from a range of tissues in rodents, monkey, and man. Common features were as follows: (a) while membrane-bound, CPT I, but not CPT II, was inhibited reversibly by malonyl-coenzyme A (CoA) and irreversibly by CoA esters of certain oxirane carboxylic acids; (6) the detergent, Tween-20, readily solubilized CPT II in active form while leaving CPT I membrane associated and catalytically functional; (c) octyl glucoside and Triton X-100 released active CPT II but caused essentially complete loss of CPT I activity. Use of [3H]tetradecylglycidyl-CoA, a covalent ligand for CPT I, yielded estimates of the enzyme's monomeric molecular size: ∼86 kDa in non-hepatic tissues and ∼90-94 kDa in liver, depending upon species. A polyclonal antibody to purified rat liver CPT II recognized a single protein in each tissue; its apparent molecular mass was ∼70 kDa in all rat tissues and ∼68 kDa in all mouse tissues as well as monkey and human liver. On Northern blot analysis a rat liver CPT II cDNA probe detected a single ∼2.5-kilobase mRNA in all rat and mouse tissues examined. The following points are emphasized. First, CPT I and II are different proteins. Second, within a species CPT II, but not CPT I, is probably conserved across tissue lines. Third, slight variations in size of both enzymes were found in different species, although, at least in the case of CPT II, significant amino acid identity exists among the various isoforms. Fourth, CPT I, unlike CPT II, requires membrane integrity for catalytic function. Finally, the strategic use of detergents provides a simple means of discriminating between the two enzyme activities.

Original languageEnglish (US)
Pages (from-to)10714-10719
Number of pages6
JournalJournal of Biological Chemistry
Volume265
Issue number18
StatePublished - Jun 25 1990

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Carnitine O-Palmitoyltransferase
Tissue
Enzymes
Liver
Rats
Coenzyme A
Membranes
Detergents
Haplorhini
Malonyl Coenzyme A
Ethylene Oxide
Mitochondria
Polysorbates
Octoxynol
Enzyme activity
Molecular mass
Carboxylic Acids
Northern Blotting
Protein Isoforms
Esters

ASJC Scopus subject areas

  • Biochemistry

Cite this

Woeltje, K. F., Esser, V., Weis, B. C., Cox, W. F., Schroeder, J. G., Liao, S. T., ... McGarry, J. D. (1990). Inter-tissue and inter-species characteristics of the mitochondrial carnitine palmitoyltransferase enzyme system. Journal of Biological Chemistry, 265(18), 10714-10719.

Inter-tissue and inter-species characteristics of the mitochondrial carnitine palmitoyltransferase enzyme system. / Woeltje, Keith F.; Esser, Victoria; Weis, Brian C.; Cox, William F.; Schroeder, James G.; Liao, Shyue Tsong; Foster, Daniel W.; McGarry, J. Denis.

In: Journal of Biological Chemistry, Vol. 265, No. 18, 25.06.1990, p. 10714-10719.

Research output: Contribution to journalArticle

Woeltje, KF, Esser, V, Weis, BC, Cox, WF, Schroeder, JG, Liao, ST, Foster, DW & McGarry, JD 1990, 'Inter-tissue and inter-species characteristics of the mitochondrial carnitine palmitoyltransferase enzyme system', Journal of Biological Chemistry, vol. 265, no. 18, pp. 10714-10719.
Woeltje KF, Esser V, Weis BC, Cox WF, Schroeder JG, Liao ST et al. Inter-tissue and inter-species characteristics of the mitochondrial carnitine palmitoyltransferase enzyme system. Journal of Biological Chemistry. 1990 Jun 25;265(18):10714-10719.
Woeltje, Keith F. ; Esser, Victoria ; Weis, Brian C. ; Cox, William F. ; Schroeder, James G. ; Liao, Shyue Tsong ; Foster, Daniel W. ; McGarry, J. Denis. / Inter-tissue and inter-species characteristics of the mitochondrial carnitine palmitoyltransferase enzyme system. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 18. pp. 10714-10719.
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abstract = "Properties of the carnitine palmitoyltransferase (EC 2.3.1.21) (CPT) enzyme system were compared in isolated mitochondria from a range of tissues in rodents, monkey, and man. Common features were as follows: (a) while membrane-bound, CPT I, but not CPT II, was inhibited reversibly by malonyl-coenzyme A (CoA) and irreversibly by CoA esters of certain oxirane carboxylic acids; (6) the detergent, Tween-20, readily solubilized CPT II in active form while leaving CPT I membrane associated and catalytically functional; (c) octyl glucoside and Triton X-100 released active CPT II but caused essentially complete loss of CPT I activity. Use of [3H]tetradecylglycidyl-CoA, a covalent ligand for CPT I, yielded estimates of the enzyme's monomeric molecular size: ∼86 kDa in non-hepatic tissues and ∼90-94 kDa in liver, depending upon species. A polyclonal antibody to purified rat liver CPT II recognized a single protein in each tissue; its apparent molecular mass was ∼70 kDa in all rat tissues and ∼68 kDa in all mouse tissues as well as monkey and human liver. On Northern blot analysis a rat liver CPT II cDNA probe detected a single ∼2.5-kilobase mRNA in all rat and mouse tissues examined. The following points are emphasized. First, CPT I and II are different proteins. Second, within a species CPT II, but not CPT I, is probably conserved across tissue lines. Third, slight variations in size of both enzymes were found in different species, although, at least in the case of CPT II, significant amino acid identity exists among the various isoforms. Fourth, CPT I, unlike CPT II, requires membrane integrity for catalytic function. Finally, the strategic use of detergents provides a simple means of discriminating between the two enzyme activities.",
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