Interaction between altered insulin and lipid metabolism in CEACAM1-inactive transgenic mice

Tong Dai; George A. Abou-Rjaily; Qusai Y. Al-Share'; Yan Yang; Mats A. Fernström; Anthony M. DeAngelis; Abraham D. Lee; Lawrence Sweetman; Antonino Amato; Marzia Pasquali; Gary D. Lopaschuk; Sandra K. Erickson; Sonia M. Najjart

doi:10.1074/jbc.M404764200

Interaction between altered insulin and lipid metabolism in CEACAM1-inactive transgenic mice

Tong Dai, George A. Abou-Rjaily, Qusai Y. Al-Share', Yan Yang, Mats A. Fernström, Anthony M. DeAngelis, Abraham D. Lee, Lawrence Sweetman, Antonino Amato, Marzia Pasquali, Gary D. Lopaschuk, Sandra K. Erickson, Sonia M. Najjart

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20 Scopus citations

Abstract

Inactivation of CEACAM1 in L-SACC1 mice by a dominant-negative transgene in liver impairs insulin clearance and increases serum free fatty acid (FFA) levels, resulting in insulin resistance. The contribution of elevated FFAs in the pathogenesis of insulin resistance is herein investigated. Treatment of L-SACC1 female mice with carnitine restored plasma FFA content. Concomitantly, it normalized insulin levels without directly regulating receptor-mediated insulin internalization and prevented glucose tolerance in these mice. Similarly, treatment with nicotinic acid, a lipolysis inhibitor, restored insulin-stimulated receptor uptake in L-SACC1 mice. Taken together, these data suggest that chronic elevation in plasma FFAs levels contributes to the regulation of insulin metabolism and action in L-SACC1 mice.

Original language	English (US)
Pages (from-to)	45155-45161
Number of pages	7
Journal	Journal of Biological Chemistry
Volume	279
Issue number	43
DOIs	https://doi.org/10.1074/jbc.M404764200
State	Published - Oct 22 2004
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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Dai, T., Abou-Rjaily, G. A., Al-Share', Q. Y., Yang, Y., Fernström, M. A., DeAngelis, A. M., Lee, A. D., Sweetman, L., Amato, A., Pasquali, M., Lopaschuk, G. D., Erickson, S. K., & Najjart, S. M. (2004). Interaction between altered insulin and lipid metabolism in CEACAM1-inactive transgenic mice. Journal of Biological Chemistry, 279(43), 45155-45161. https://doi.org/10.1074/jbc.M404764200

Dai, T, Abou-Rjaily, GA, Al-Share', QY, Yang, Y, Fernström, MA, DeAngelis, AM, Lee, AD, Sweetman, L, Amato, A, Pasquali, M, Lopaschuk, GD, Erickson, SK & Najjart, SM 2004, 'Interaction between altered insulin and lipid metabolism in CEACAM1-inactive transgenic mice', Journal of Biological Chemistry, vol. 279, no. 43, pp. 45155-45161. https://doi.org/10.1074/jbc.M404764200

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title = "Interaction between altered insulin and lipid metabolism in CEACAM1-inactive transgenic mice",

abstract = "Inactivation of CEACAM1 in L-SACC1 mice by a dominant-negative transgene in liver impairs insulin clearance and increases serum free fatty acid (FFA) levels, resulting in insulin resistance. The contribution of elevated FFAs in the pathogenesis of insulin resistance is herein investigated. Treatment of L-SACC1 female mice with carnitine restored plasma FFA content. Concomitantly, it normalized insulin levels without directly regulating receptor-mediated insulin internalization and prevented glucose tolerance in these mice. Similarly, treatment with nicotinic acid, a lipolysis inhibitor, restored insulin-stimulated receptor uptake in L-SACC1 mice. Taken together, these data suggest that chronic elevation in plasma FFAs levels contributes to the regulation of insulin metabolism and action in L-SACC1 mice.",

author = "Tong Dai and Abou-Rjaily, {George A.} and Al-Share', {Qusai Y.} and Yan Yang and Fernstr{\"o}m, {Mats A.} and DeAngelis, {Anthony M.} and Lee, {Abraham D.} and Lawrence Sweetman and Antonino Amato and Marzia Pasquali and Lopaschuk, {Gary D.} and Erickson, {Sandra K.} and Najjart, {Sonia M.}",

year = "2004",

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doi = "10.1074/jbc.M404764200",

language = "English (US)",

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AU - Dai, Tong

AU - Abou-Rjaily, George A.

AU - Al-Share', Qusai Y.

AU - Yang, Yan

AU - Fernström, Mats A.

AU - DeAngelis, Anthony M.

AU - Lee, Abraham D.

AU - Sweetman, Lawrence

AU - Amato, Antonino

AU - Pasquali, Marzia

AU - Lopaschuk, Gary D.

AU - Erickson, Sandra K.

AU - Najjart, Sonia M.

PY - 2004/10/22

Y1 - 2004/10/22

N2 - Inactivation of CEACAM1 in L-SACC1 mice by a dominant-negative transgene in liver impairs insulin clearance and increases serum free fatty acid (FFA) levels, resulting in insulin resistance. The contribution of elevated FFAs in the pathogenesis of insulin resistance is herein investigated. Treatment of L-SACC1 female mice with carnitine restored plasma FFA content. Concomitantly, it normalized insulin levels without directly regulating receptor-mediated insulin internalization and prevented glucose tolerance in these mice. Similarly, treatment with nicotinic acid, a lipolysis inhibitor, restored insulin-stimulated receptor uptake in L-SACC1 mice. Taken together, these data suggest that chronic elevation in plasma FFAs levels contributes to the regulation of insulin metabolism and action in L-SACC1 mice.

AB - Inactivation of CEACAM1 in L-SACC1 mice by a dominant-negative transgene in liver impairs insulin clearance and increases serum free fatty acid (FFA) levels, resulting in insulin resistance. The contribution of elevated FFAs in the pathogenesis of insulin resistance is herein investigated. Treatment of L-SACC1 female mice with carnitine restored plasma FFA content. Concomitantly, it normalized insulin levels without directly regulating receptor-mediated insulin internalization and prevented glucose tolerance in these mice. Similarly, treatment with nicotinic acid, a lipolysis inhibitor, restored insulin-stimulated receptor uptake in L-SACC1 mice. Taken together, these data suggest that chronic elevation in plasma FFAs levels contributes to the regulation of insulin metabolism and action in L-SACC1 mice.

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Interaction between altered insulin and lipid metabolism in CEACAM1-inactive transgenic mice

Abstract

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