Interaction between lactobacilli, bacterial vaginosis-associated bacteria, and HIV Type 1 RNA and DNA genital shedding in U.S. and Kenyan Women

Caroline Mitchell, Jennifer E. Balkus, David Fredricks, Congzhou Liu, Jennifer McKernan-Mullin, Lisa M. Frenkel, Christina Mwachari, Amneris Luque, Susan E. Cohn, Craig R. Cohen, Robert Coombs, Jane Hitti

Research output: Contribution to journalReview article

40 Citations (Scopus)

Abstract

Bacterial vaginosis has been associated with genital HIV-1 shedding; however, the effect of specific vaginal bacterial species has not been assessed. We tested cervicovaginal lavage from HIV-1-seropositive women for common Lactobacillus species: L. crispatus, L. jensenii, and seven BV-associated species: BVAB1, BVAB2, BVAB3, Leptotrichia, Sneathia, Megasphaera, and Atopobium spp. using quantitative PCR. We used linear and Poisson regression to evaluate associations between vaginal bacteria and genital HIV-1 RNA and DNA. Specimens from 54 U.S. (310 visits) and 50 Kenyan women (137 visits) were evaluated. Controlling for plasma viral load, U.S. and Kenyan women had similar rates of HIV-1 RNA (19% of visits vs. 24%; IRR=0.95; 95% CI 0.61, 1.49) and DNA shedding (79% vs. 76%; IRR=0.90; 0.78, 1.05). At visits during antiretroviral therapy (ART), the likelihood of detection of HIV-1 RNA shedding was greater with BVAB3 (IRR=3.16; 95% CI 1.36, 7.32), Leptotrichia, or Sneathia (IRR=2.13; 1.02, 4.72), and less with L. jensenii (IRR=0.39; 0.18, 0.84). At visits without ART, only L. crispatus was associated with a lower likelihood of HIV-1 RNA detection (IRR=0.6; 0.40, 0.91). Vaginal Lactobacillus species were associated with lower risk of genital HIV-1 shedding, while the presence of certain BV-associated species may increase that risk.

Original languageEnglish (US)
Pages (from-to)13-19
Number of pages7
JournalAIDS Research and Human Retroviruses
Volume29
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

Bacterial Vaginosis
Lactobacillus
HIV-1
RNA
Bacteria
DNA
Leptotrichia
Megasphaera
Therapeutic Irrigation
Viral Load
insulin receptor-related receptor
Linear Models
Polymerase Chain Reaction
Therapeutics

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Cite this

Interaction between lactobacilli, bacterial vaginosis-associated bacteria, and HIV Type 1 RNA and DNA genital shedding in U.S. and Kenyan Women. / Mitchell, Caroline; Balkus, Jennifer E.; Fredricks, David; Liu, Congzhou; McKernan-Mullin, Jennifer; Frenkel, Lisa M.; Mwachari, Christina; Luque, Amneris; Cohn, Susan E.; Cohen, Craig R.; Coombs, Robert; Hitti, Jane.

In: AIDS Research and Human Retroviruses, Vol. 29, No. 1, 01.01.2013, p. 13-19.

Research output: Contribution to journalReview article

Mitchell, C, Balkus, JE, Fredricks, D, Liu, C, McKernan-Mullin, J, Frenkel, LM, Mwachari, C, Luque, A, Cohn, SE, Cohen, CR, Coombs, R & Hitti, J 2013, 'Interaction between lactobacilli, bacterial vaginosis-associated bacteria, and HIV Type 1 RNA and DNA genital shedding in U.S. and Kenyan Women', AIDS Research and Human Retroviruses, vol. 29, no. 1, pp. 13-19. https://doi.org/10.1089/aid.2012.0187
Mitchell, Caroline ; Balkus, Jennifer E. ; Fredricks, David ; Liu, Congzhou ; McKernan-Mullin, Jennifer ; Frenkel, Lisa M. ; Mwachari, Christina ; Luque, Amneris ; Cohn, Susan E. ; Cohen, Craig R. ; Coombs, Robert ; Hitti, Jane. / Interaction between lactobacilli, bacterial vaginosis-associated bacteria, and HIV Type 1 RNA and DNA genital shedding in U.S. and Kenyan Women. In: AIDS Research and Human Retroviruses. 2013 ; Vol. 29, No. 1. pp. 13-19.
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abstract = "Bacterial vaginosis has been associated with genital HIV-1 shedding; however, the effect of specific vaginal bacterial species has not been assessed. We tested cervicovaginal lavage from HIV-1-seropositive women for common Lactobacillus species: L. crispatus, L. jensenii, and seven BV-associated species: BVAB1, BVAB2, BVAB3, Leptotrichia, Sneathia, Megasphaera, and Atopobium spp. using quantitative PCR. We used linear and Poisson regression to evaluate associations between vaginal bacteria and genital HIV-1 RNA and DNA. Specimens from 54 U.S. (310 visits) and 50 Kenyan women (137 visits) were evaluated. Controlling for plasma viral load, U.S. and Kenyan women had similar rates of HIV-1 RNA (19{\%} of visits vs. 24{\%}; IRR=0.95; 95{\%} CI 0.61, 1.49) and DNA shedding (79{\%} vs. 76{\%}; IRR=0.90; 0.78, 1.05). At visits during antiretroviral therapy (ART), the likelihood of detection of HIV-1 RNA shedding was greater with BVAB3 (IRR=3.16; 95{\%} CI 1.36, 7.32), Leptotrichia, or Sneathia (IRR=2.13; 1.02, 4.72), and less with L. jensenii (IRR=0.39; 0.18, 0.84). At visits without ART, only L. crispatus was associated with a lower likelihood of HIV-1 RNA detection (IRR=0.6; 0.40, 0.91). Vaginal Lactobacillus species were associated with lower risk of genital HIV-1 shedding, while the presence of certain BV-associated species may increase that risk.",
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AU - Frenkel, Lisa M.

AU - Mwachari, Christina

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